山西医科大学学报2025，Vol.56Issue(2)：142-150,9.DOI:10.13753/j.issn.1007-6611.2025.02.005
和肝汤颗粒对功能性消化不良大鼠肠道菌群和TLR4信号通路的影响
Effects of Hegantang granules on intestinal microbiota and TLR4 signaling pathway in rats with functional dyspepsia
摘要
Abstract
Objective To explore the effect of Hegantang granules on functional dyspepsia(FD)and its mechanism based on gut mi-crobiota and the Toll-like receptor 4(TLR4)signaling pathway.Methods Eighteen 6-week-old SD rats were randomly divided into normal group,model group,and Hegantang group,with 6 rats in each group.FD model was established using tail-clamping stress in model group and Hegantang group.After modeling,the rats in Hegantang group were given 0.918 g crude drug/100 g body weight by gavage for 7 d.After the intervention,duodenum tissues and cecal contents were collected.The transcription level of TLR4 gene was detected by quantitative polymerase chain reaction(qPCR).TLR4 protein expression was assessed by Western blotting.And the ex-pression location of TLR4 was observed using immunofluorescence(IF)and immunohistochemistry(IHC).Additionally,16S rRNA and liquid chromatograph-mass spectrometer(LC-MS)were used to analyze the gut microbiota and metabolites in the cecal contents.Results Compared to normal group,TLR4 protein expression was significantly increased in model group(P<0.01).In comparison with model group,TLR4 expression was downregulated in Hegantang group(P<0.05).IF and IHC results revealed that TLR4 ex-pressed in both lamina propria and epithelium of the duodenum in all groups.Compared with normal group,positive staining was en-hanced in model group(P<0.01),while it was reduced in Hegantang group compared with model group(P<0.01).However,there was no significant difference in TLR4 gene transcription level among the groups(P>0.05).Compared with normal group,the abundance of Verrucomicrobiota was increased in model group(P<0.05),while the abundance of Firmicutes was decreased(P<0.05).Compared with model group,the abundance of Bacteroidota was increased in Hegantang group(P<0.05),and the abundance of Verrucomicro-biota was downregulated(P<0.05).Additionally,there were differences in the levels of metabolites such as n-γ-glutamylglutamine among the groups(P<0.05).Conclusion The expression of TLR4 in the duodenum is upregulated in FD,and there are abnormal changes in intestinal microbiota and metabolites.Hegantang granules may improve FD by regulating the changes of intestinal micro-biota and metabolites,and downregulating TLR4 expression in the duodenum.关键词
和肝汤颗粒/功能性消化不良/肠道菌群/十二指肠/Toll样受体4Key words
Hegantang granules/functional dyspepsia/intestinal flora/duodenum/Toll-like receptor 4分类
临床医学引用本文复制引用
赵静怡,连博,陆梦馨,郑金粟,朱宏勋,高剑虹..和肝汤颗粒对功能性消化不良大鼠肠道菌群和TLR4信号通路的影响[J].山西医科大学学报,2025,56(2):142-150,9.基金项目
北京市级中西医协同"旗舰"科室建设项目 （）
朝阳区区级中医重点专科培育类项目 （）
北京市医管局培育计划项目(PZ2022003) （PZ2022003）
