山西医科大学学报2025，Vol.56Issue(2)：151-157,7.DOI:10.13753/j.issn.1007-6611.2025.02.006
载5-ASA的尤特奇S100包衣氧化铁-壳聚糖纳米复合物对小鼠溃疡性结肠炎的治疗作用及机制
Therapeutic effects and mechanism of Eudragit S100 coated iron oxide-chitosan nanocomposites with 5-aminosalicylic acid on ulcerative colitis in mice Table 1 Criteria of DAI score
摘要
Abstract
Objective To investigate the effect and its underlying mechanism of Eudragit S100 coated iron oxide-chitosan nanocom-posites of 5-aminosalicylic acid on ulcerative colitis in mice.Methods Six to eight weeks old male C57BL/6 mice were randomly assigned to four groups:normal control group,TNBS model group,5-ASA group and ES-IOCS/5-ASA group.The mouse ulcerative colitis model was established by TNBS.The mice in 5-ASA group and ES-IOCS/5-ASA group were gavaged with the corresponding drugs for seven consecutive days after modeling.Then the mice were killed.The disease activity index(DAI)was evaluated,the serum levels of IL-1β,IL-6 and TNF-α were detected by ELISA,and the colon tissues of mice were stained with hematoxylin and eosin(HE).Western blot was used to determine the protein levels of NLRP3,apoptosis-associated speck-like protein(ASC),and IL-1β in colon tissues.Results Compared with normal control group,the body weight was significantly decreased in TNBS model group(P<0.001),the disease activity index(DAI)was significantly increased(P<0.001),the colon length was shortened(P<0.001),the patho-logical injury score was increased(P<0.001),the levels of IL-1β,IL-6 and TNF-α in serum were significantly increased(P<0.05),and the expressions of NLRP3,ASC,and IL-1β were upregulated(P<0.001).Compared with TNBS model group,the DAI score was reduced in ES-IOCS/5-ASA group(P<0.001),the colon length was enlarged(P<0.001),DAI was decreased(P<0.001),the expres-sions of pro-inflammatory cytokines IL-1β,TNF-α in serum were downregulated(P<0.05),and the expression of NLRP3 inflamma-some in colon tissues was decreased(P<0.001).However,5-ASA had poor efficacy on ulcerative colitis in mice.Conclusion ES-IOCS/5-ASA may alleviate TNBS-induced ulcerative colitis in mice through the inhibition of NLRP3 inflammasome activation.关键词
5-氨基水杨酸/三硝基苯磺酸/溃疡性结肠炎/NLRP3炎症小体/纳米复合物/小鼠Key words
5-amino salicylic acid/trinitrobenzene sulphonic acid/ulcerative colitis/NLRP3 inflammasome/nanocomposites/mice分类
临床医学引用本文复制引用
张丹丹,王燕,赵冉,谭学明..载5-ASA的尤特奇S100包衣氧化铁-壳聚糖纳米复合物对小鼠溃疡性结肠炎的治疗作用及机制[J].山西医科大学学报,2025,56(2):151-157,7.基金项目
东南大学附属中大医院江北院区面上项目(JB202005M) （JB202005M）
