山西医科大学学报2025，Vol.56Issue(2)：175-181,7.DOI:10.13753/j.issn.1007-6611.2025.02.009
柱前衍生化顶空进样气相色谱法测定蚂蚁及其制剂中蚁酸的含量
Determination of formic acid in ants and their preparations by gas chromatography with pre-column derivatization and headspace injection
摘要
Abstract
Objective To establish a method for determining the content of formic acid in ants and their preparations.Methods The content of formic acid in ants and their preparations was determined by gas chromatography(GC).Formic acid and isopropanol were esterified to form isopropyl formate.The headspace injection method was adopted,with FID(hydrogen flame ionization detector)as the detector,85℃equilibrated for 30 min,and headspace injection.The chromatographic separation conditions were as follows:the initial temperature of the column was 50℃,the program temperature ramping rate was 20℃/min,the carrier gas flow rate of the chromatographic column was 2.5 mL/min,and the split ratio was 10∶1.The methodology of the established headspace injection gas chromatography method was investigated.Results Formic acid had a good linear relationship in the range of 20-600 μg(R2=0.999 2),the average recovery rate was 95.2%-95.9%,the intermediate precision RSD was 2.0%,the repeatability RSD was 2.9%,the detec-tion limit was 0.007 0 mg/g,and the quantification limit was 0.020 mg/g.Conclusion This method is rapid,simple and accurate,and it is suitable for the determination of formic acid in ants and their preparations.关键词
蚁酸/蚂蚁制剂/柱前衍生化/顶空进样/气相色谱法/含量测定/方法学验证Key words
formic acid/ant preparations/pre-column derivatization/headspace injection/gas chromatography/content determination/methodological validation分类
药学引用本文复制引用
梁卜文,申国华,董培智..柱前衍生化顶空进样气相色谱法测定蚂蚁及其制剂中蚁酸的含量[J].山西医科大学学报,2025,56(2):175-181,7.基金项目
食品药品安全防控山西省重点实验室基金资助项目(202204010931006) （202204010931006）
