时珍国医国药2025，Vol.36Issue(1)：1-8,8.DOI:10.70976/j.1008-0805.SZGYGY-2025-0101
基于RIPK1/RIPK3/MLKL途径探讨温肺降浊方对VaD大鼠坏死性凋亡的影响
The Wenfei Jiangzhuo Formula exerts protective effects on VaD model rats by inhibiting nec-rotic apoptosis through attenuating the RIPK1/RIPK3/MLKL pathway
摘要
Abstract
Objective To investigate whether the Wenfei Jiangzhuo Formula inhibits RIPK1/RIPK3/MLKL pathway to reduce necrotic apoptosis and thus exerts cerebroprotective effects on VaD model rats.Methods Healthy male SD rats were randomly divided into the Sham group,the model group,the inhibitor group,and the low-dose,medium-dose and high-dose groups of the Wenfei Jiangzhuo Formula;except for the Sham group,the other groups were prepared as VaD models,and each group was gavaged with the corresponding drugs after successful modeling,and the tests were carried out after 3 weeks of treatment.Behavior,morphological damage in hippocam-pal CA1 area and changes in Nissl vesicles were observed in each group.Serum concentrations of IL-1β、IL-6 and TNF-α were de-tected by ELISA;and the expression of RIPK1/RIPK3/MLKL pathway proteins and mRNAs were detected by Western blot and qRT-PCR.Results Compared with the Sham group,rats in the model group and each intervention group had prolonged evasion latency(P<0.05)and shortened number of crossing platforms(P<0.05);hippocampal neurons had a sparse morphology,with ruptured morpholo-gy of some cells,edema,shrunken nuclei,and severe deletion of Nissl vesicles with structural vacuoles;and the expression of IL-1β,IL-6,and TNF-α in the serum was elevated(P<0.05);The expression of RIPK1/RIPK3/MLKL pathway proteins and mRNA in rat hippocampal tissues was elevated(P<0.05);compared with the model group,the evasion latency of rats in the inhibitor group and the high-dose group of the Wenfei Jiangzhuo Formula was shortened(P<0.05),and the number of traversals across the plateau was in-creased(P<0.05);the number of hippocampal neurons increased,the cells were arranged tightly,and their morphology gradually regu-larized,and their structure more intact;the expression of IL-1β,IL-6 and TNF-α in serum decreased(P<0.05);the expression of RIPK1/RIPK3/MLKL pathway protein and mRNA in hippocampal tissue decreased(P<0.05).Conclusion The Wenfei Jiangzhuo Formula can inhibit the RIPK1/RIPK3/MLKL pathway to reduce necrotic apoptosis,thus improving the cognitive condition of the brain and exerting cerebroprotective effects.关键词
温肺降浊方/坏死性凋亡/RIPK1/RIPK3/MLKL信号通路/血管性痴呆/神经元凋亡/作用机制Key words
The Wenfei Jiangzhuo Formula/Necroptosis/RIPK1/RIPK3/MLKL signaling pathway/Vascular dementia/Neu-ronal apoptosis/Mechanisms of action分类
医药卫生引用本文复制引用
张鼎,陈炜,秦红玲,张玲,向军军,王开龙,姚春,胡跃强..基于RIPK1/RIPK3/MLKL途径探讨温肺降浊方对VaD大鼠坏死性凋亡的影响[J].时珍国医国药,2025,36(1):1-8,8.基金项目
国家自然科学基金(82260904) （82260904）
广西中医药大学"桂派杏林拔尖人才"计划(04B2300807 （）
04B2300907) （）
国家中医药管理局高水平重点学科-中医内科学(ZYYZDXK-2023166) （ZYYZDXK-2023166）
广西中医药大学"岐黄工程"高层次人才团队(202410) （202410）
广西中医药重点学科建设项目(GZXK-Z-20-13) （GZXK-Z-20-13）
