时珍国医国药2025，Vol.36Issue(1)：17-24,8.DOI:10.70976/j.1008-0805.SZGYGY-2025-0103
脉络宁口服液对动脉硬化性闭塞症大鼠的影响
Exploring the effect of Mailuoning oral liquid on rats with arteriosclerotic occlusion
摘要
Abstract
Objective To explore the effect of Mailuoning oral liquid(MOL)on arteriosclerotic occlusion(ASO)rats by regulating the NFAT5/NLRP3 signaling pathway.Methods The rat model of ASO was established by femoral artery injury combined with high-fat diet feeding for 75 days.The ASO rats were randomly divided into the model group,the MOL low-dose and high-dose groups,Mailuoning injection(MI),the atorvastatin calcium group and the KRN2(NFAT5 inhibitor)group,and each group with 5 rats was administered continuously for 14 days.HE staining was used to observe the pathological changes of femoral artery.Kits were used to detect the levels of blood lipid,UA and HMGB1 in plasma of ASO rats.Q-PCR was adopted to detect the mRNA expressions of the indicators related to aortic endothelial dysfunction,hepatic lipid metabolism disorder and inflammation.Western blot(WB)was utilized to detect the protein expressions of the NFAT5/NLRP3 signaling pathway in the liver tissues of ASO rats.Results Results showed that the intimas of femoral arteries were proliferated and the vascular lumina of femoral arteries were narrowed in ASO rats.The levels of TC,TG,LDL-C,UA and HMGB1 in plasma and the mRNA expressions of HMGB1,RAGE,MCP-1,NLRP3,NFAT5,MMP9,MKP-1 and ACLS1 in the aorta and liver tissues of ASO rats were significantly higher than those of control group(P<0.01).The NFAT5/NLRP3 signaling path-way in the liver tissues of ASO rats were activated(P<0.01).The intimal injury of femoral artery in the MOL and KRN2 rats were a-meliorated.The increase of the above indicators in plasma,aorta and liver tissues in ASO rats were inhibited after treated by MOL and KRN2(P<0.05,P<0.01).Conclusion MOL may treat ASO by reducing endothelial injury,regulating liver lipid metabolism and blood lipid level,and reducing inflammatory reaction.The mechanism may be closely related to the inhibition of the NFAT5/NLRP3 sig-naling pathway.关键词
动脉硬化性闭塞症大鼠模型/脉络宁口服液/NFAT5/NLRP3信号通路/炎症/脂代谢Key words
Arterioscleroticocclusion rat model/Mailuoning oral liquid/NFAT5/NLRP3 signaling pathway/Inflammation/Lip-id metabolism分类
医药卫生引用本文复制引用
来兴兆,范玲玲,黄素清,黄聪聪,李剑,谭宁华..脉络宁口服液对动脉硬化性闭塞症大鼠的影响[J].时珍国医国药,2025,36(1):17-24,8.基金项目
金陵药业股份有限公司企业合作项目(校合2020-中003 （）
校合2023-中067) （）
