时珍国医国药2025，Vol.36Issue(1)：62-66,5.DOI:10.70976/j.1008-0805.SZGYGY-2025-0109
金水清合剂对于IgA肾病大鼠TRPV1水平的影响
Exploring the effect of Jinshuiqing Mixture on the levels of TRPV1 in IgA nephropathy rats
摘要
Abstract
Objective To explore the correlation between IgA nephropathy and heat syndrome,and the effect of Jinshuiqing Mixture(JSQM)on the level of TRPV1 in IgA nephropathy rats.Methods Fifty-four SD rats were randomly divided into the normal group,the control group,and the treatment group.An IgA nephropathy model was established in the control and treatment groups.Based on the control group,the treatment group was gavaged with JSQM and euthanized.Renal tissues were taken to observe the pathological changes of glomeruli by using fluorescence immunoassay.Western blot(WB)was used to detect the expression of TRPV1 protein in the hypothal-amus and renal tissues.Results Compared with the normal group,the levels of serum creatinine(Cr),blood urea nitrogen(BUN),and aspartate aminotransferase(AST)in the control and treatment groups were significantly increased(P<0.0001),while the concentra-tions of Cr,BUN,and AST in the treatment group were lower than those in the control group(P<0.05;P<0.01;P<0.0001).Com-pared with the normal group,the deposition of IgA immune complexes was observed in the glomeruli of rats in the control and treatment groups.Among them,the control group had relatively more IgA immune complexes.Compared with the normal group,the concentration of TRPV1 protein in the hypothalamus and kidneys of the control and treatment groups rats was significantly increased(P<0.01).Com-pared with the control group,the concentration of TRPV1 protein in the hypothalamus and kidneys of the treatment group was decreased(P<0.01).Conclusion The heat syndrome objectively exists in IgA nephropathy rats,and JSQM can significantly improve renal dam-age in IgA nephropathy rats whose mechanism of action may be related to its ability to down-regulate the concentration of TRPV1 protein in the hypothalamus and renal tissue.关键词
IgA肾病/金水清合剂/TRPV1Key words
IgA nephropathy/Jinshuiqing Mixture/TRPV1分类
医药卫生引用本文复制引用
吴聪,刘洋,余远瞻,崔荣华..金水清合剂对于IgA肾病大鼠TRPV1水平的影响[J].时珍国医国药,2025,36(1):62-66,5.基金项目
湖北省自然科学基金项目(2022CFD156) （2022CFD156）
湖北省中医药管理局中医药重点学科建设项目(P-20230316-1137) （P-20230316-1137）
