天津师范大学学报（自然科学版）2025，Vol.45Issue(1)：13-21,9.DOI:10.19638/j.issn1671-1114.20250103
嗜水气单胞菌非编码RNA的功能研究
Function study of non-coding RNA of Aeromonas hydrophila
摘要
Abstract
To reveal the function of non-coding RNA(sRNA)of Aeromonas hydrophila,which is a species of prokaryote,A.hydrophila ATCC 7966 strain was taken as experimental material,and transcriptome sequencing technology was used for transcriptome analysis.sRNA was predicted,the sRNA gene deletion mutant of A.hydrophila(ΔsRNA)was constructed by homologous recombination,and the physiological characteristics and pathogenicity of ΔsRNA were analyzed.The results showed that the growth status of ΔsRNA and wild type strain were almost the same,and there was no difference of motility between WT and ΔsRNA.The results of hemolysis test showed that there was obvious hemolysis in both strains.The growth inhibition of the two strains was the same in hypertonic environment.Compared with WT strain,the biofilm forming ability and protease activity of ΔsRNA were significantly lower(P＜0.01),and ΔsRNA strain was more sensitive to penicillin and erythromycin.Due to the loss of sRNA gene,the virulence of ΔsRNA was reduced.关键词
嗜水气单胞菌/转录组测序/非编码RNA/基因敲除/致病性Key words
Aeromonas hydrophila/transcriptome sequencing/non-coding RNA/gene knockout/pathogenicity分类
生物学引用本文复制引用
王嘉璐,蔡彤璇,陈甜梦,李娜,刘瑶瑶,赵宝华,刘东..嗜水气单胞菌非编码RNA的功能研究[J].天津师范大学学报（自然科学版）,2025,45(1):13-21,9.基金项目
河北省自然科学基金资助项目(C2019205044) （C2019205044）
河北省高等学校科学技术研究项目(ZD2018070) （ZD2018070）
河北省教育厅优秀青年基金资助项目(YQ2014026) （YQ2014026）
河北师范大学重点基金资助项目(L2016Z03). （L2016Z03）
