天津师范大学学报（自然科学版）2025，Vol.45Issue(1)：27-33,7.DOI:10.19638/j.issn1671-1114.20250105
高寒区裸石弃渣场植被恢复中覆土厚度对土壤细菌群落组成和功能的影响
Effects of covering soil thickness of waste rock land on the composition and function of bacterial community at alpine vegetation restoration
摘要
Abstract
To clarify the composition and function of bacterial community responding to the different covering soil thickness of waste rock land in alpine area,a recovery field experiment involving different covering soil thickness was conducted in Mangkang County,Tibet.Plant samples were collected by the quadrat sampling method,and soil samples by the double diago-nal line were collected after five years.The soil properties,composition and function of bacterial community,and the relation-ship between them at different covering soil thickness were analyzed.The results showed that:① The aboveground biomass of plant increased with covering soil thickness and the content of nitrogen and phosphorus was the highest in covering soil 20 cm.② Proteobacteria was the dominant group,and the relative abundance of Bacteroidota,Firmicutes and Acidobacteriota were affected by covering soil thickness.③ Covering soil thickness did not significantly affect α diversity of bacterial community,but significantly affected β diversity of bacterial community.β diversity is the highest in covering soil 20 cm.④ FAPROTAX analy-sis result showed that denitrification of bacterial community was enhanced in covering soil 10 cm.RAD analysis results showed that soil total phosphorus content was positively relative with the denitrification of bacterial community.⑤ Fermentation and chemoheterotrophy of bacterial community were improved in covering soil 20 cm,which was negatively relative with soil available nitrogen and total nitrogen content.Relative abundance of Firmicutes also increased in covering soil 20 cm,which increased nitrogen metabolism.⑥ Nitrification of bacterial community was promoted in covering soil 30 cm.The relative abundance of Bacteroidota also increased in covering soil 30 cm,which could decay cellulose and then support the carbon for microbial growth.In conclusion,covering soil thickness above 20 cm is an economical and effective method to restore the vegetation in alpine region according to the cost of covering soil,biomass of plant and changing in composition and function of bacterial community.关键词
弃渣场/植被恢复/覆土厚度/细菌群落组成/细菌群落功能Key words
waste rock land/vegetation restoration/covering soil thickness/composition of bacterial community/function of bacterial community分类
生物学引用本文复制引用
孙荣,吴建波,王鑫喜,陈凡,王小丹..高寒区裸石弃渣场植被恢复中覆土厚度对土壤细菌群落组成和功能的影响[J].天津师范大学学报（自然科学版）,2025,45(1):27-33,7.基金项目
第二次青藏高原综合科学考察研究项目(SQ2019QZKK2004) （SQ2019QZKK2004）
中国华能集团公司总部科技项目(HNKJ20-H23). （HNKJ20-H23）
