天津师范大学学报（自然科学版）2025，Vol.45Issue(1)：34-43,10.DOI:10.19638/j.issn1671-1114.20250106
不同电子供体底物对自养型硫氧化菌同步厌氧除硫脱氮过程的影响
Influence of different electron donor substrates on simultaneous anaerobic sulfide and nitrate removal of autotrophic sulfur-oxidizing bacteria
摘要
Abstract
The influence of different electron donor substrates on simultaneous anaerobic sulfide and nitrate removal of auto-trophic sulfur-oxidizing bacteria was studied using a segmented progressive wastewater sulfide removal process(SPWSRP).In the experiment,if the ratio of sulfide to ni trate(S/N)and pH value of the wastewater system were controlled well,the system reac-tion was predictable.When sulfide was used as the electron donor substrate,and if the S/N ratio was set at 3∶2 and the influent pH value at 6.9±0.1,the maximum removal percentages of sulfide and nitrate in the wastewater were 99.5％and 99.9％respectively,and their corresponding maximum volumetric removal load rates were 4.32 kg/(m3·d)and 1.11 kg/(m3·d)respectively.The sub strates'metabolic pathways were relatively long and complicated,and the corresponding types of metabolites were diverse.Then the metabolites covered several metabolic intermediates such as S0,S2O32-and NO2-and end products including SO42-and N2.In addition,the possible reaction mechanisms were analyzed based on typical experimental phenomena such as reaction cessation,and variation of ammonium concentration in the wastewater.When thiosulfate was used as the electron donor sub-strate,and the S/N ratio was set at 5∶8 and the influent pH value at 7.1±0.1,the maximum removal efficiencies of thiosulfate and nitrate in the reaction system were separately 99.8％and 99.9％,and their respective maximum volumetric loads were 8.66 kg/(m3·d)and 3.04 kg/(m3·d)respectively.The substrates'metabolic pathway was relatively short and simple,with rela tively few intermediates and end products being generated.The detectable intermediate was only NO2-,and the end products included SO42-and N2.While elemental sulfur and other nitrogen-oxides were not detected.The metabolites including intermediates and end products were similar to those with sulfide as electron donor substrate,but with huge differences.Two systems with different electron donor as substrate had different reaction paths,diverse reaction mechanisms and different reasons for reaction cessation.From the view of metabolic process,the thiosulfate metabolic pathway was not a shortened version of the sulfide metabolic way.Based on this,choosing appropriate electron donor substrate in wastewater treatment could not only achieve the goal of sulfide and nitrate removal but also prevent side reactions from occurring.关键词
同步厌氧除硫脱氮/废水除硫工艺/电子供体底物/硫氮比/代谢途径/代谢产物Key words
simultaneous anaerobic sulfide and nitrate removal/wastewater sulfide removal process/electron donor substrate/ratio of sulfide to nitrate/metabolic pathway/metabolites分类
生物学引用本文复制引用
吴新世,朱广斌,张万良..不同电子供体底物对自养型硫氧化菌同步厌氧除硫脱氮过程的影响[J].天津师范大学学报（自然科学版）,2025,45(1):34-43,10.基金项目
高校产学研联合创新项目(H20220409). （H20220409）
