天津中医药大学学报2025，Vol.44Issue(2)：123-130,8.DOI:10.11656/j.issn.1673-9043.2025.02.06
虎杖苷与马鞭草苷改善环磷酰胺诱导小鼠免疫抑制的作用比较
Comparison of polydatin and verbenalin in ameliorating cyclophosphamide-induced immunosuppression in mice
摘要
Abstract
[Objective]Comparison of the ameliorative effects of polydatin and verbenalin on immune function in cyclophosphamide(CY)-induced immunosuppressed mice.[Methods]Twenty-four male BALB/C mice were randomly divided into four groups:control group,model group,polydatin group and verbenalin group,with six mice in each group.The control group and model group were given pure water gavage,while the polydatin and verbenalin groups were given polydatin and verbenalin gavage treatment,respectively.After 11 days,the blood was collected,and serum immunoglobulin G(IgG)and immunoglobulin M(IgM)were measured by the enzyme-linked immunosorbent assay(ELIS A).The thymus and spleen were isolated and weighed,then the thymus and spleen indices were calculated.The pathological structures of the liver,spleen,and thymus were analyzed by HE staining.RT-PCR was used to detect interleukin-2(IL-2)and interleukin-4(IL-4)expression in spleen.CD4+and CD8+T cell counts were assessed by flow cytometry.The proliferation of splenic B cells was evaluated using the CCK-8 assay.[Results]Consistently,after polydatin or verbenalin administration,the index of the spleen and the level of IL-2 both were significantly upregulated.The pathological alteration was also much improved.The lipopolysaccharide(LPS)-induced proliferation of B cells was much enhanced.In addition,after polydatin administration,the body weight and thymus index increased significantly.The levels of IgM and IgG and the counts of CD4+and CD8+T cells were also upregulated.[Conclusion]Polydatin and verbenalin have certain immunomodulatory effects,and the immunomodulatory effect of polydatin is better than that of verbenalin.关键词
虎杖苷/马鞭草苷/环磷酰胺/免疫抑制/免疫功能Key words
polydatin/verbenalin/cyclophosphamide/immunosuppressed/immunological function分类
中医学引用本文复制引用
刘秀苹,赵薇,徐兴娣,闫慧敏,张静,王梦,孙娜,苗琳..虎杖苷与马鞭草苷改善环磷酰胺诱导小鼠免疫抑制的作用比较[J].天津中医药大学学报,2025,44(2):123-130,8.基金项目
国家重点研发计划项目(2023YFC3504302) （2023YFC3504302）
天津中医药大学"雏鹰计划"科研项目(XJS2023027) （XJS2023027）
天津市教委科研计划项目(2023KJ139). （2023KJ139）
