摘要
Abstract
[Objective]Establishing UPLC specific chromatogram of Artemisia capillaris,combining a quantitative analysis of multi-components by single marker(QAMS)and chemical pattern recognition analysis to evaluate the quality of Artemisia capillaris dispensing granules.[Methods]The high-performance liquid chromatography was adopted to analysis the characteristic chromatograms of 17 batches Artemisia capillaris standard decoction and 6 batches dispensing granules,and the common pattern was established,and the similarity evaluation,hierarchical cluster analysis(HCA),principal component analysis(PCA),orthogonal partial least squares method-discriminant analysis(OPLS-DA)were applied to evaluate difference.The QAMS method was established,chlorogenic acid was selected as the internal reference for determining the relative correction factor f of chlorogenic acid and neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A,isochlorogenic acid B,isochlorogenic C,the content of each indicator component was calculated,and the results were compared with those of the external standard method to judge the accuracy of the method.[Results]The results of specific chromatogram of 17 batches Artemisia capillaris standard decoction and 6 batches dispensing granules showed that 11 common peaks were determined and 7 components were identified.Through similarity evaluation,HCA,PCA and OPLS-DA,the difference components between Artemisia capillaris standard decoctions and dispensing granules were screened.The relative deviation of content results of each indicator component in Artemisia capillaris standard decoction and dispensing granules by the QAMS and external standard method was less than 5.0％,the data without significant difference.[Conclusion]The established specific chromatogram of Artemisia capillaris and the QAMS have been investigated by methodological,the method is sensitive,accurate,simple and easy to implement,combined with chemical pattern recognition,which can be used to analyze the differences between Artemisia capillaris standard decoction and dispensing granules,provide support for the control of production process and quality evaluation of dispensing granules.关键词
茵陈标准汤剂/配方颗粒/特征图谱/一测多评/化学模式识别Key words
Artemisia capillaris standard decoction/dispensing granules/specific chromatogram/quantitative analysis of multi-components by single marker(QAMS)/chemical pattern recognition分类
中医学
