天津中医药大学学报2025，Vol.44Issue(2)：140-144,5.DOI:10.11656/j.issn.1673-9043.2025.02.08
腹部推拿对单纯性肥胖模型大鼠摄食量及结肠PYY与GLP-1表达影响
Effects of abdominal acupressure on food intake and colonic PYY and GLP-1 expression in rats with simple obesity model
摘要
Abstract
[Objective]To explore the effect of abdominal nudging on the expression of colonic PYY and GLP-1 in the model of simple obese rats,and to analyze its possible mechanism of action.[Methods]24 SD male rats were randomly divided into 3 groups:blank group,model group and nudging group,8 rats each.The model group and the nudging group were given homemade high-fat feed to establish simple obesity animal model.In the nudging group,abdominal nudging was applied to the abdomen[rubbing Zhongwan(RN12)and pushing Renmai(CV)]for 20 min/times,1 day for 14 d.The model group was only given the same time restraint as that of the nudging group.The blank group was not modeled or intervened.At the end of the intervention,the body mass,food intake,body length and Lee's index of the rats in the three groups were examined to evaluate the changes in their physiological indexes.Enzyme-l inked immunosorbent assay(ELISA)was used to examine the levels of Peptide YY(PYY)and Glucagon Like Peptide-1(GLP-1)in the intestines of rats.HE staining was applied to observe the morphological and structural changes of intestinal colon tissue.[Results]Compared with the rats before and after their own,the body mass and food intake of the nudging group decreased(P＜0.01);compared with the model group,the fat coefficient of the nudging group decreased(P＜0.01),and the expression of PYY and GLP-1 was elevated(P＜0.01);the HE test showed that the structure of the colonic glands of the model group was disorganized,with crypts shrinking and deforming,and the number of cup cells was small,and there was infiltration of chronic inflammatory cells.After abdominal acupressure,the morphology of colon tissue tended to return to the normal state.[Conclusion]Abdominal acupressure may improve the expression of intestinal PYY and GLP-1,regulate the gastrointestinal function,inhibit appetite,and create a negative energy balance of intake-consumptionin purely obese rats to achieve the effect of fat reduction and weight loss.关键词
腹部推拿/单纯性肥胖/PYY/GLP-1/摄食量/体质量Key words
abdominal acupressure/simple obesity/PYY/GLP-1/food intake/body weight分类
中医学引用本文复制引用
黄偲崟,谭涛,李华南,马菲,安成飞,边明真,宁静..腹部推拿对单纯性肥胖模型大鼠摄食量及结肠PYY与GLP-1表达影响[J].天津中医药大学学报,2025,44(2):140-144,5.基金项目
国家级非物质文化遗产项目——津沽脏腑推拿项目(IX-2)(12000023P13YBDA100102) （IX-2）
天津市名中医传承工作室建设(2024年)项目(tjmzy2405) （2024年）
天津市中医药管理局中医中西医结合科研课题项目(2021005) （2021005）
国家中医药管理局推拿学高水平重点学科 （）
国家区域中医(推拿)诊疗中心项目(20200424). （推拿）
