中国土壤与肥料Issue(1)：53-61,9.DOI:10.11838/sfsc.1673-6257.24174
基于宏基因组学探究碳氮联合激发Arthrobacter参与关键碳代谢促进植物生长的研究
Metagenomics reveals that carbon and nitrogen combination enhances Arthrobacter's participation in critical carbon metabolism to promote plant growth
摘要
Abstract
Soil carbon and nitrogen nutrients play a crucial role in plant growth.To investigate the effects of carbon and nitrogen on crop growth,a field experiment was conducted in the yellow soil region of central Guizhou.Four treatments were established,including control(CK),glucose(G),urea(U),and glucose+urea(GU).The impacts of exogenous carbon and nitrogen on crop growth,soil microbial community,and metabolism were assessed.The GU treatment significantly increased the fresh weight,dry weight,and stem diameter of rapeseed compared to other treatments(P<0.05),and the plant height of rapeseed was also the highest in the GU treatment.The soil pH content of the G,U and GU treatments was lower than that of the CK treatment,while the soil total nitrogen,alkali-hydrolyzable nitrogen,and organic matter content were higher than those of the CK treatment.The predominant species of soil microbial community in the GU treatment was Arthrobacter,suggesting its potential importance in the GU treatment.At KEGG Pathway levels 2,nitrogen metabolism and carbohydrate metabolism were prominent.Among the nitrogen cycle metabolism pathways,the nitrogen fixation metabolism pathway dominated in the G treatment,while the nitrification metabolism pathway dominated in the U treatment.In the carbon cycle metabolism pathway,there were 18 significantly different metabolic pathways between the GU treatment and the other three treatments,with 6,11 and 3 pathways in CK,G and U,respectively.In the GU treatment,18 significantly different metabolic pathways were identified,with the metabolic genes mainly originating from Arthrobacter.These genes included glucose kinase glk(K00845),ribose-5-phosphate isomerase B rpiB(K01808),1,6-diphosphofructokinase glpX(K02446),6-phospho-3-hexulose isomerase hxlB(K08094),and phosphofructokinase pfk and pfp(K21071).Structural equation modeling demonstrated a direct and significant impact of carbon cycle metabolic genes on rapeseed growth and yield(P<0.001).This study laid the groundwork for investigating the influence of carbon and nitrogen on crop growth from a microbial perspective and provided theoretical support for research on crop yield improvement.关键词
外加碳氮/Arthrobacter/氮循环/碳循环/结构方程模型Key words
in addition to carbon and nitrogen/Arthrobacter/nitrogen cycle/carbon cycle/structural equation modeling引用本文复制引用
刘超,秦仕华,闫茹,李仕蓉,许巧玲,乔志伟..基于宏基因组学探究碳氮联合激发Arthrobacter参与关键碳代谢促进植物生长的研究[J].中国土壤与肥料,2025,(1):53-61,9.基金项目
贵州省科技计划(黔科合基础ZK[2023]一般024) （黔科合基础ZK[2023]一般024）
贵州省教育厅高等学校科学研究项目青年项目(黔教技[2022]327,黔教技[2022]331). （黔教技[2022]327,黔教技[2022]331）
