西北林学院学报2025，Vol.40Issue(2)：1-8,19,9.DOI:10.3969/j.issn.1001-7461.2025.02.01
XsbZIP17调控文冠果抗旱性的功能
Function of XsbZIP17 in Regulating the Drought Resistance of Yellowhorn
摘要
Abstract
The objective of this study was to explore the effect of XsbZIP17 on the drought resistance of yel-lowhorn(Xanthoceras sorbifolium)for increasing the understanding of the drought resistance regulatory network of yellowhorn,and for selecting yellowhorn varieties with stronger drought resistance.Bioinforma-tics methods was adopted to analyze the structural features of XsbZIP17 gene,and the 35S∷XsbZIP17-eYFP fusion protein was constructed to observe the subcellular localization of XsbZIP17.The expression pattern of XsbZIP17 in five tissues of yellowhorn,including roots,stems,young leaves,mature leaves,and flowers,were clarified through qRT-PCR.XsbZIP17 was heterologously overexpressed by Agrobacterium-mediated genetic technology to obtain transgenic Arabidopsis thaliana.We observed the growth pheno-types of wild type(WT)and transgenic plants under drought stress,measured their physiological indica-tors of proline,malondialdehyde,and chlorophyll,and expression levels of drought resistance related genes.The research has found that XsbZIP17 possesses the conserved structural domain bZIP-HY5-like charac-teristic of the bZIP family and is located in the nucleus.XsbZIP17 had a higher expression level in the stems and roots of yellowhorn,and its expression was strongly induced by drought and PEG treatment.Under drought stress,the survival rate of XsbZIP17 overexpression(XsbZIP17-OE)lines was significant-ly higher than that of the WT,and the proline and chlorophyll contents of XsbZIP17-OE lines were also higher than that of the WT,while the MDA content of XsbZIP17-OE lines was lower than that of the WT.And the expression levels of genes related to drought response in transgenic plants significantly increased.In summary,XsbZIP17 plays an important role in regulating the drought resistance of yellowhorn.XsbZ-IP17 increases the proline content of transgenic plants under drought conditions to maintain osmotic pres-sure balance,reduces the degree of plant plasma membrane peroxidation,and increases the chlorophyll con-tent to maintain normal photosynthesis,thereby improving plant drought resistance.关键词
文冠果/XsbZIP17/抗旱性/生理响应/基因表达Key words
yellowhorn/XsbZIP17/drought resistance/physiological response/gene expression分类
农业科技引用本文复制引用
班卓,杨莹莹,许慧慧,刘肖娟,毕泉鑫,王利兵..XsbZIP17调控文冠果抗旱性的功能[J].西北林学院学报,2025,40(2):1-8,19,9.基金项目
国家自然科学基金项目(31870594). （31870594）
