西北林学院学报2025，Vol.40Issue(2)：64-72,83,10.DOI:10.3969/j.issn.1001-7461.2025.02.07
红桦花粉生活力及贮藏特性研究
Pollen Viability and Storage Characteristics of Betula albosinensis
摘要
Abstract
Using Betula albosinensis pollen as a material,we explored the optimal medium for its in vitro culture germination,and compared the accuracy of TTC staining,I-KI staining,and magenta acetate meth-od in determining the viability of the pollen;meanwhile,we discussed the storage effect of the pollen in terms of its water content,whether the container was vacuumed or not,and the storage temperature.The results showed that the suitable medium and culture temperature for in vitro culture of the pollen were 10 g/L agar+15％sucrose+250 mg/L boric acid and 30 ℃,10 h,the activity was the highest,and TTC stai-ning was suitable for the rapid detection of the viability of the pollen in the field,and the best effect was achieved when the pH of the staining solution was 7.0.The optimal storage temperature of the pollen were-80 to-40 ℃,and B.albosinensis pollen was favorable for storage after dried and vacuumed.The study clarifies the characteristics and storage characteristics of B.albosinensis pollen,and provides a theoretical basis for the hybrid breeding and germplasm innovation of B.albosinensis.关键词
红桦/花粉离体萌发/花粉贮藏Key words
Betula albosinensis/pollen germination in vitro/pollen storage分类
农业科技引用本文复制引用
李宁,杨喜彬,王旭红,孟苏星,彭少兵..红桦花粉生活力及贮藏特性研究[J].西北林学院学报,2025,40(2):64-72,83,10.基金项目
国家重点研发项目"红桦育种群体构建及新种质创建"(2021YFD2200304-3). （2021YFD2200304-3）
