西北林学院学报2025，Vol.40Issue(2)：93-102,10.DOI:10.3969/j.issn.1001-7461.2025.02.10
海南岛2种森林类型地下水微生物研究
Microorganism of Groundwater in Two Forest Types in Hainan Island
摘要
Abstract
The objective of this study was to explore the composition and diversity of groundwater microbial communities and environmental driving factors in different forests,so as to provide basic data for the study of tropical forests.Metagenomic sequencing technology was used to analyze the composition and diversity of groundwater microbial communities and environmental factors in two types of forests,tropical rainforest and rubber forest occurring in Hainan Island.The results showed that 1)there were obvious differences in the composition of groundwater microbial communities between tropical rainforest and rubber forest(P＜0.001),and the proportion of archaeal community was higher than that of tropical rainforest,while that of Euryarchaeota was significantly lower than that of tropical rainforest(P＜0.05).Among the bacterial communities,Proteobacteria was slightly higher in rubber forest than in tropical rainforest,while Bacte-roidetes was lower than that in tropical rainforest(P＜0.05).In the fungal community,Ascomycota was significantly higher than that in tropical rainforest(P＜0.05),while Mucoromycota was significantly lower than in tropical rainforest(P＜0.001).2)The α diversity of groundwater microorganisms in tropical rain-forests was higher than that in rubber forests.The α diversity of archaeal communities from tropical rain-forests to rubber forests significantly reduced(P＜0.001),the α diversity of bacterial communities signifi-cantly reduced(P＜0.05),and the α diversity of fungal communities significantly increased(P＜0.05).Principal coordinate analysis(PCoA)and similarity analysis revealed the β diversity of groundwater micro-organisms,significant differences were observed between rubber forests and tropical rainforests in Hainan Island(archaea:R=0.666,P=0.001;bacteria:R=0.519,P=0.001;fungi:R=0.384,P=0.001).3)The total dissolved solids,altitude,and redox potential of groundwater were the most important environmental factors affecting archaeal,bacterial,and fungal communities,respectively.Atmospheric pressure,bicarbon-ate,and altitude had a significant impact on the microbial beta diversity of groundwater,with altitude being the most significant environmental factor.关键词
热带森林/地下水/微生物多样性/环境因子Key words
tropical forest/groundwater/microbial diversity/environmental factor分类
农业科技引用本文复制引用
庞通,孙瑞,兰国玉,魏亚情,杨川,符庆茂..海南岛2种森林类型地下水微生物研究[J].西北林学院学报,2025,40(2):93-102,10.基金项目
国家自然科学基金项目(32271603) （32271603）
海南省自然科学基金高层次人才项目(320RC733) （320RC733）
中央级公益性科研院所基本科研业务费专项(1630022022003). （1630022022003）
