西南林业大学学报2025，Vol.45Issue(1)：26-33,8.DOI:10.11929/j.swfu.202312041
云南樟基质添加对牛樟芝生长特性的影响
Effects of Cinnamomum glanduliferum Matrix Addition on the Growth Characteristics of Antrodia cinnamomea
摘要
Abstract
To explore the effect of Cinnamomum glanduliferum substrate addition on the growth and physiological and biochemical characteristics of Antrodia cinnamomea.Potato agar medium(PDA)was supple-mented with 10 concentrations(0.125-64 g/L)of C.glanduliferum branches,leaves and their mixtures and cul-tured at constant temperature for 30 days,and the differences in colony diameter,mycelial density,colony color,colony growth potential,mycelial growth rate,growth index,biomass,superoxide dismutase(SOD)activity,and total triterpene(TT)content were analyzed.The findings indicated that under the condition of adding C.glanduliferum branches in PDA medium,the growth characteristics and biomass of A.cinnamomea were higher when the addition concentration was 1-8 g/L,and the SOD activity and TT content of A.cinnamomea were signi-ficantly higher when the addition concentration was 2-4 g/L and 2 g/L,respectively;under the condition of adding young leaves of C.glanduliferum to PDA medium,its growth characteristics and biomass were higher when the concentration was 1-4 g/L and 1-16 g/L,respectively.When the concentration was 0.5-2 g/L,its SOD activity and TT content were significantly higher than those of the control;the growth characteristics,biomass,SOD activ-ity and TT content were kept at a high level when the concentration of C.glanduliferum branches and leaves mix-ture addition was 2-16 g/L,4--16 g/L,16-32 g/L and 8-32 g/L,respectively.It follows that adding 4 g/L of C.glanduliferum young leaf as the substrate has the best promoting effect on the growth indicators and biomass of A.cinnamomea,with a colony diameter of 83.40 mm,a mycelial growth rate of 2.78 mm/d,and a biomass of 0.23 g;adding 2 g/L of C.glanduliferum branches matrix significantly increased the SOD activity of A.cinnamomea;the addition of 16 g/L mixed substrate of C.glanduliferum branches and leaves significantly promoted the total triter-pene content of A.cinnamomea.关键词
牛樟芝/云南樟/固体培养/SOD/总三萜Key words
Antrodia cinnamomea/Cinnamomum glanduliferum/solid culture/SOD/total triterpene分类
农业科技引用本文复制引用
宝元朵,黄兴连,杨志娟,武自强,张颖,郑元..云南樟基质添加对牛樟芝生长特性的影响[J].西南林业大学学报,2025,45(1):26-33,8.基金项目
国家自然科学基金项目(32160736)资助 （32160736）
云南省农业基础研究联合专项重点项目(202101BD070001-020)资助 （202101BD070001-020）
云南省基础研究计划面上项目(202101AT070044)资助 （202101AT070044）
"兴滇英才支持计划"青年人才专项项目(XDYC-QNRC-2022-0245)资助. （XDYC-QNRC-2022-0245）
