西南林业大学学报2025，Vol.45Issue(1)：44-54,11.DOI:10.11929/j.swfu.202404007
基于单细胞转录组的水稻稀有气孔细胞类型鉴定
Identification of Rare Stomatal Cell Types in the Single-cell Transcriptome of Rice
摘要
Abstract
To explore the regulatory mechanism of development and movement of grass stomata,on the basis of the primary clustering,the study successfully obtained independent and stable clusters of guard cells and subsi-diary cells from single-cell transcriptome data in rice leaves by sub-clustering analysis of all the obtained epiderm-al cells when the resolution value of sub-clustering was set at 2.6,combined with more marker genes.They con-sist of 12 guard cells and 17 subsidiary cells,respectively.GO enrichment analysis revealed the biological pro-cesses of late stomatal development such as"microtubule-based movement","signal transduction"and"response to stimulus".Gene regulatory network analysis revealed genes such as OsDOF11,OsMAPK2,OsNAC75,OsAB-CG4,OsERF52 and OsSWEET2b and their potential interaction networks.The results of this study provide a methodological reference for the identification of rare cell types in plants,and also provides new marker genes for the study of stomatal cells in other grasses.关键词
稀有细胞类型/气孔/水稻/单细胞转录组Key words
rare cell type/stomata/rice/single-cell transcriptome分类
农业科学引用本文复制引用
刘开燕,孙桂玲,郭振华..基于单细胞转录组的水稻稀有气孔细胞类型鉴定[J].西南林业大学学报,2025,45(1):44-54,11.基金项目
国家自然科学基金面上项目(32270716)资助. （32270716）
