西南林业大学学报2025，Vol.45Issue(1)：68-77,10.DOI:10.11929/j.swfu.202401067
外源NO对盐胁迫下八角金盘叶片生理特性及解剖结构的影响
Effects of Exogenous NO on the Physiological Characteristics and Anatomical Structure of Fatsia japonica Leaves Under Salt Stress
摘要
Abstract
To study the effects of exogenous nitric oxide(NO)on physiological characteristics and the ana-tomical structure of Fatsia japonica leaves under salt stress(NaCl).The half-year-old Fatsia japonica was treated with 1.2%NaCl solution by pot experiment,and exogenous NO donor(15 mL,0.1 mmol/L Nitroprusside Sodium)was sprayed on the leaf surface for a total of 30 processing days.Photosynthetic parameters and physiological in-dexes were measured at 0,15(mild)and 30 d(moderate)after salt treatment,and observed the anatomical struc-ture of leaves.The results were as follows:Fatsia japonica was able to adapt to mild salt stress by increasing the activity of antioxidant enzymes,accumulating Pro,adding thickness to the cuticle and main veins.However,mod-erate salt stress significantly reduced the photosynthesis,RWC,photosynthetic pigment,and SS content in the leaves of Fatsia japonica,leading to a significant accumulation of MDA(malondialdehyde)and disorganized mesophyll tissue,with a marked thinning of leaf thickness.The application of exogenous NO(nitric oxide)effect-ively mitigated the reduction in photosynthesis under salt stress,significantly increased leaf RWC,and the con-tent of photosynthetic pigments,SS,and SP,enhanced CAT and APX activities,and markedly reduced MDA levels.In addition,exogenous NO also reduced the thickness of the upper epidermis and cuticle layer,promoted mesophyll growth,increased the ratio of palisade to spongy tissue,and enhanced the salt tolerance of Fatsia ja-ponica.In summary,Fatsia japonica has a certain degree of salt tolerance,and the application of exogenous NO can effectively alleviate the damage caused by moderate salt stress to Fatsia japonica leaves and enhance its res-istance to salt stress.关键词
八角金盘/外源一氧化氮/盐胁迫/生理特性/叶片解剖结构Key words
Fatsia japonica/exogenous nitric oxide/salt stress/physiological characteristic/leaf anatomical structure分类
园艺学与植物营养学引用本文复制引用
胡星,胡纪龙,张敏,刘娇,黄晓霞..外源NO对盐胁迫下八角金盘叶片生理特性及解剖结构的影响[J].西南林业大学学报,2025,45(1):68-77,10.基金项目
云南省高层次人才"青年拔尖人才"项目(YNWR-QNBJ-2020-222)资助. （YNWR-QNBJ-2020-222）
