西南林业大学学报2025，Vol.45Issue(1)：174-180,7.DOI:10.11929/j.swfu.202404054
白星花金龟幼虫粪中除氨菌的筛选与鉴定
Screening and Identification of Ammonia-removing Bacteria in the Feces of Protaetia brevitarsis Larvae
摘要
Abstract
To isolate and screen microorganisms with ammonia removal effect from the feces of the larvae of Protaetia brevitarsis,and to provide microbial resources for the conversion and utilization of organic waste,2 strains of ammonia-removing bacteria N1 and N2 were screened from the feces of the larvae of P.brevitarsis by means of enrichment and domestication,preliminary screening and re-screening.The strains were identified by morphological,physiological and biochemical,and 16SrDNA molecular identification.The physiological charac-teristics and growth curve of the strain were determined.The effects of single strain and compound strain on NH3 release within 25 days were determined by boric acid absorption Kjeldahl method.The findings indicated that both N1 and N2 showed obvious ammonia removal ability,and the ammonia removal effect of the compound strain was higher than that of the single strain.N1 was identified as Ochrobactrum tritici,and N2 was identified as Ochrobactrum sp.The growth curves of N1 and N2 had their own characteristics.The 2 strains grew best at 30℃-37℃pH=5-9.The 2 strains isolated from the larvae of P.brevitarsis can effectively reduce the release of NH3,and the ammonia removal effect of the 2 strains is higher than that of the single strain,which can be used as an effective ammonia removal microorganism during the fermentation of organic waste.关键词
白星花金龟/幼虫虫粪/微生物/鉴定/除氨菌Key words
Protaetia brevitarsis/larval feces/microorganism/identification/ammonia-removing bacteria分类
生物科学引用本文复制引用
刘业超,王庆雷,刘雪英,赵鹏武,晁开瑞,张居宇,姚湘,陈萌,张燕如..白星花金龟幼虫粪中除氨菌的筛选与鉴定[J].西南林业大学学报,2025,45(1):174-180,7.基金项目
内蒙古自治区自然科学基金项目(2020BS03014)资助 （2020BS03014）
国家自然科学基金项目(32160372)资助 （32160372）
内蒙古自治区林学"双一流"建设项目(DC2100001171)资助 （DC2100001171）
内蒙古自治区林业科技推广示范项目资助. （）
