湖北科技学院学报（医学版）2025，Vol.39Issue(2)：107-110,4.DOI:10.16751/j.cnki.2095-4646.2024082010
小檗碱对脂多糖诱导的胸主动脉环损伤的保护作用
The Protective Effect of Berberine on Lipopolysaccharids-induced Thoracic Aortic Ring Injury
摘要
Abstract
Objective To investigate the effect and mechanism of berberine(BBr)on lipopolysaccharide(LPS)-induced impairment of thoracic aortic rings.Methods The Kunming mice thoracic aortic rings were quickly isolated and suspended in a Multi Myograph System.The isolated rings were pretreated with different concentration of BBR(0.1～10μmol/L)for 30 min,and then treated with 1 μg/mL LPS for 2 hours.The changes of isometric tension were recorded induced by LPS.The Nitric Oxide(NO)and malondialdehyde(MDA)levels of thoracic aorta ring homogenate were measured by the kits.Aortic rings isolated from mice were preincubated with L-nitroarginine methyl methyl(L-NAME)1 mmol/L or Tempol 1 mmol/L for 30 minutes.The above drug treatment steps were repeated,as well as the record of isometric tension.Results LPS dramatically decreased the content of NO and increased the level of MDA.LPS dramatically impaired acetyl choline(Ach)-induced endothelium-dependent relaxation(all P＜0.05).After the treatment of BBr,the above situation was alleviated in a dose-dependent manner.L-NAME blocked the function of BBr.Tempol had no effect on the function of BBr.However,sodium nitroprusside(SNP)-induced endothelium-inde-pendent relaxation was not altered in all groups.Conclusion BBr plays a protective role in LPS-induced oxidative stress injury of endothelial dysfunction in aortic arteries isolated from Kunming mice ex vivo by regulating NO signaling.关键词
小檗碱/氧化应激/一氧化氮/血管损伤Key words
Berberine/Oxidative stress/NO/Vascular injury分类
药学引用本文复制引用
李其会,赵银利,赵辛元..小檗碱对脂多糖诱导的胸主动脉环损伤的保护作用[J].湖北科技学院学报（医学版）,2025,39(2):107-110,4.基金项目
湖北省中医药科研项目(ZY2023F149) （ZY2023F149）
湖北科技学院药学重点学科专项科研项目(2019-20YZ01) （2019-20YZ01）
湖北科技学院2019年度五官学院专项科研基金项目(2020XZ41) （2020XZ41）
