湖北科技学院学报（医学版）2025，Vol.39Issue(2)：116-120,125,6.DOI:10.16751/j.cnki.2095-4646.2024082206
BDG联合mNGS在非HIV感染儿童耶氏肺孢子菌肺炎中的诊断价值
Diagnostic Value of BDG Combined with mNGS in Non-HIV Infected Children with PJP
摘要
Abstract
Objective To evaluate the diagnostic value of serum 1,3-beta-D glucan(BDG)combined with metagenomic next-generation sequencing(mNGS)for Pneumocystis jirovecii pneumonia(PJP)in with non-human immunodeficiency virus(HIV)infected children.Methods A retrospective collection was conducted on 21 PJP children and 41 non PJP children from 4 hospitals.The clinical characteristics of PJP patients were summarized,and the diagnostic efficacy of PJP was evaluated using ser-um 1,3-beta-D glucan(BDG)combined with BALF-mNGS.Receiver operating characteristic(ROC)curves were drawn,and spearman analysis was used to analyze the correlation between the two group.Results The area under the BALF-mNGS curve of was 0.907(95％CI 0.772～0.963),and the area under the curve of serum BDG was 0.869(95％CI0.743～0.971).The optimal cut-off values for BALF-mNGS and serum BDG to distinguish whether PJP was infected were 12 sequence numbers(sensitivity 83.2％,specificity 94.3％)and 64.6 ng/L(sensitivity 79.2％,specificity 93.1％),respectively.Spearman correlation analysis showed a significant correlation between the mNGS sequences and serum BDG(r=0.718,P＜0.001).When the number of mNGS sequences in BALF was≥12 and serum BDG was≥64.6 ng/L,the sensitivity was 95.2％(95％CI 74.1％-99.8％),the specificity was 85.3％(95％CI 70.1％-93.9％),the positive predictive value was 76.9％(95％CI 55.9％～90.2％),and the negative predictive value was 97.2％(95％CI 83.8％-99.9％)for joint diagnosis.Conclusion Early BALF mNGS com-bined with serum BDG is helpful for the diagnosis of PJP children with non-HIV infected.关键词
耶氏肺孢子菌肺炎/宏基因组二代测序/诊断效能/儿童Key words
Pneumocystis jiroveci pneumonia/Metagenomic next-generation sequencing/Diagnostic efficacy/Children分类
临床医学引用本文复制引用
安渤宇,吴春风..BDG联合mNGS在非HIV感染儿童耶氏肺孢子菌肺炎中的诊断价值[J].湖北科技学院学报（医学版）,2025,39(2):116-120,125,6.基金项目
吉林省卫生健康科技能力提升项目(2021LC069) （2021LC069）
