信阳师范学院学报（自然科学版）2025，Vol.38Issue(1)：17-23,7.DOI:10.3969/j.issn.2097-583X.2025.01.003
SSR标记引物及其在识别茶树"信阳10号"上的应用
SSR marker primer and its application on identification of tea plant "Xinyang 10"
摘要
Abstract
Aiming at the problem that "Xinyang 10" is not easily identified among tea germplasm based on phenotype or biochemical components,SSR molecular marker primers were developed to genotype tea germplasm based on "Shuchazao (SCZ)" genomic data,and the identification of "Xinyang 10" among tea germplasm was studied. Eight tea germplasm randomly selected from 188 tea germplasm were genotyped with randomly selected SSR markers and the results showed that the SSR marker primer SCZSSR03 was polymorphic and was selected. Using the primer to genotype 188 tea germplasm,the DNA banding pattern of "Xinyang 10"was significantly different from those of 187 other tea germplasm. The SCZSSR03 molecular marker primer screened can effectively identify "Xinyang 10",and the molecular marker technology for identifying "Xinyang 10" was established based on the SSR marker,which can provide germplasm identification technology for tea germplasm resource research,genetic improvement and protection by utilizing "Xinyang 10" variety.关键词
茶树/种质识别/简单序列重复/"信阳10号"Key words
Camellia sinensis L./germplasm identification/simple sequence repeat(SSR)/"Xinyang 10"分类
农业科学引用本文复制引用
毛光志,邢锋,李梦笛,朱国金,陈震,衡双平,张伟,周棋赢,袁红雨,江昌俊..SSR标记引物及其在识别茶树"信阳10号"上的应用[J].信阳师范学院学报（自然科学版）,2025,38(1):17-23,7.基金项目
国家自然科学基金项目(22208276) （22208276）
科技部重点专项项目(2021YFD1601100) （2021YFD1601100）
河南省中央引导地方科技发展资金项目(Z20231811160) （Z20231811160）
河南省高等学校重点科研项目(22A210023) （22A210023）
信阳师范大学"南湖学者奖励计划"青年项目(2020B12) （2020B12）
