信阳师范学院学报（自然科学版）2025，Vol.38Issue(1)：111-116,6.DOI:10.3969/j.issn.2097-583X.2025.01.015
丹参LBD26基因的克隆和表达分析
Cloning and expression analysis of LBD26 gene in Salvia miltiorrhiza
摘要
Abstract
To understand the biological role of Salvia miltiorrhiza LBD26 transcription factor in the accumulation of medicinal ingredients,the SmLBD26 gene was cloned;the sequence characteristics,homologous evolution and promoter element analysis were investigated using bioinformatic methods;The expression specificity of the tissues and the expression level under hormonal and stress treatments were tested by quantitative real time polymerase chain reaction(qRT-PCR). Sequence characterization results showed that the total length of SmLBD26 CDS was 702 bp,encoding 233 amino acids and the SmLBD26 protein was an unstable,hydrophilic nucleoprotein. The secondary structure of the SmLBD26 protein was dominated by random coil,followed by α-helix,extended strand and β-turn. The Blastp and phylogenetic analysis found that it contained a LOB domain,belonged to the Class Ⅱ LBD subfamily and it had high homology and a close evolutionary relationship with LBD proteins from other species. Expression analysis results showed that the SmLBD26 gene was expressed in roots,stems,leaves and flowers,and the expression level was high in roots. The expression of SmLBD26 was down regulated after treatment with abscisic acid,auxin,MeJA,4 ℃ and cadmium,indicating that the SmLBD26 gene was involved in the response of Salvia miltiorrhiza to hormones and different stresses.关键词
SmLBD26蛋白/基因克隆/生物信息学分析/表达分析Key words
SmLBD26 protein/gene cloning/bioinformatics analysis/expression analysis分类
农业科技引用本文复制引用
李娟,肖正涛,洪礼伟,于新波,于莹,杨奕樱..丹参LBD26基因的克隆和表达分析[J].信阳师范学院学报（自然科学版）,2025,38(1):111-116,6.基金项目
贵州省教育厅青年科技人才成长项目(黔教合KY[2021]193) （黔教合KY[2021]193）
国家自然科学基金地区基金项目(82160719) （82160719）
贵州中医药管理局中医药、民族医药科学技术项目(QZYY-2022-013) （QZYY-2022-013）
贵州中医药大学博士启动基金项目([2020]30) （[2020]30）
