亚太传统医药2025，Vol.21Issue(3)：33-39,7.DOI:10.11954/ytctyy.202503006
不同基原大黄药材鉴别:以大黄对照提取物为参照
Identification of Different Original Rhubarb Medicinal Materials,Using Rhubarb Reference Extract as a Reference
摘要
Abstract
Objective:To identify three different origins of rhubarb by thin layer chromatography and high performance liquid chromatography,using the extractive reference substance of rhubarb as a reference.Methods:Used cyclohexane-ethyl acetate-formic acid(10∶6∶0.1)and trichloromethane-methanol-water(8:3:0.3)as developing agents to an-alyze the anthraquinone glycoside components and anthraquinone glycoside components in different origins of rhubarb;High performance liquid chromatography was used to analyze the fingerprint spectra of rhubarb with different origins,the chromatographic column was Kromasil Ci8(4.6mm × 250mm,5 μm),the mobile phase is acetonitrile-0.1％phos-phoric acid,gradient elution,column temperature of 30 ℃,injection amount of 10 μL,and detection wavelength of 269 nm.Results:Under these chromatographic conditions,there were significant differences in the anthraquinone glyco-side components and anthraquinone glycoside components among the three different origins of rhubarb.It can be clearly distinguished.Conclusion:The standardized prepared rhubarb control extract has good applicability in the identification of the origin of rhubarb medicinal materials.关键词
掌叶大黄/唐古特大黄/药用大黄/对照提取物/薄层色谱/指纹图谱Key words
Rheum Palmatum L./Rheum Tanguticum Maxim.ex Balf./Rheum Officinale Baill./Extractive Reference Substance/Thin Layer Chromatography/Fingerprint分类
医药卫生引用本文复制引用
黄国凯,陈海燕,郭隆钢,刘潇潇,吴垠,赵岳锐,窦浩,吴群悦..不同基原大黄药材鉴别:以大黄对照提取物为参照[J].亚太传统医药,2025,21(3):33-39,7.基金项目
广东省药品监督管理局科技创新项目(2022TDZ01) （2022TDZ01）
