亚太传统医药2025，Vol.21Issue(3)：46-52,7.DOI:10.11954/ytctyy.202503008
蜂王浆药材质量标准研究
Quality Standard of Royal Jelly Medicine
摘要
Abstract
Objective:To improve the quality standard of royal jelly.Methods:The qualitative identification of proline acid,lysine and histidine in royal jelly was carried out by thin-layer chromatography(TLC);the moisture content of royal jelly was determined by drying at 80℃,and the content of 10-hydroxydec-2-enoic acid was determined by high-performance liquid chromatography(HPLC)on an Agilent ZORBAX SB-C18 column(250 mm × 4.6 mm,5 μm)with acetonitrile-water(22∶78)as a mobile phase;flow rate of 1 mL/min;detection wavelength of 212 nm;column temper-ature:30 ℃;the injection volume of the control was 5 μL and that of the sample was 10 μL.Results:The TLC plots of proline acid,lysine and histidine were clear,and there was no interference with the negative control.The linear relation-ship between the sample volume of 10-hydroxy-2-decenoic acid and the peak area was good in the range of 0.010 4 mg/mL-1.040 2 mg/mL,respectively.The RSD of the precision,stability,and reproducibility tests were all less than 2.0％,and the average recoveries of the mascot were 102.0％with the RSD of 1.8％,and the average content of the 13 batches of samples was 1.906％.Conclusion:The established method is highly specific and reproducible,and can be used to con-trol the quality of royal jelly.关键词
蜂王浆/质量标准/TLC鉴别/水分/高效液相色谱法Key words
Royal Jelly/Quality Standard/TLC Identification/Moisture/High Performance Liquid Chromatography分类
医药卫生引用本文复制引用
韩生兰,李永鹏,乔亚玲,宋霞,刘亚蓉..蜂王浆药材质量标准研究[J].亚太传统医药,2025,21(3):46-52,7.基金项目
青海省"昆仑英才高端创新创业人才"计划(青人才字[2021]13号) （青人才字[2021]13号）
