亚太传统医药2025，Vol.21Issue(3)：58-62,5.DOI:10.11954/ytctyy.202503010
基于"性状学-生物效应-超微量扫描-指纹图谱"的两面针及其五种混伪品的系统鉴别
Systematic Identification of Zanthoxylum Nitidum and its Five Adulterants Based on"Morphology-Biological Effect-Ultra-Trace Scanning-Fingerprint"
摘要
Abstract
Objective:The identification of Zanthoxylum nitidum(Roxb.)DC.and its five adulterants Zanthoxylum dissitum Hemsl,Zanthoxylum armatum,Zanthoxylum bungeanum,Toddalia asiatica(L.)Lam.,and Mahonia bealei(Fort.)Carr will be conducted based on the"morphology-biological effect-ultra-trace scanning-fingerprint"method.Methods:The powders of 6 medicinal materials were observed under an optical microscope.The maximum absorption wavelength of the extracts was determined through ultra-micro UV scanning.Their inhibitory activities against acetyl-cholinesterase,α-glucosidase,and DPPH radical scavenging were detected using an enzyme-labeled instrument.Finger-print was obtained through high-performance liquid chromatography.Results:Identifying exocuticle patterns and color dis-tinguishes Zanthoxylum nitidum from Toddalia asiatica and Mahonia bealei.The rough or smooth xylem distinguishes Zan-thoxylum nitidum from Zanthoxylum armatum and Zanthoxylum bungeanum.In microscopic identification,Mahonia bealei was distinguished based on the crystalline sheath fiber and pericycle fiber.The IC50 magnitude was used to distinguish Zan-thoxylum dissitum in biological activity identification.The maximum absorption wavelength obtained through ultra-micro UV scanning and fingerprint distinguishes between Zanthoxylum armatum,Zanthoxylum bungeanum,Toddalia asiatica,and Ma-honia bealei.Conclusion:This study has comprehensively identified 6 medicinal materials,ensuring the quality of Zanthoxyli Radix,and provided a guarantee for the clinically rational application of Zanthoxylum nitidum.It laid a foundation for the fu-ture identification,quality evaluation,and biological effect evaluation of Zanthoxylum nitidum.关键词
两面针/鉴定/生物活性/乙酰胆碱酯酶抑制剂/超微量紫外扫描Key words
Zanthoxylum Nitidum/Identification/Biological Activity/Acetylcholinesterase Inhibitors/Ultra-Micro UV Scanning分类
医药卫生引用本文复制引用
张佳诺,陈月华,王文瑀,赵明月,张慧,康廷国,宋慧鹏..基于"性状学-生物效应-超微量扫描-指纹图谱"的两面针及其五种混伪品的系统鉴别[J].亚太传统医药,2025,21(3):58-62,5.基金项目
辽宁省教育厅基本科研项目(JYTQN2023455,JYTMS20231826) （JYTQN2023455,JYTMS20231826）
辽宁中医药大学自然科学类青年项目(2021LZY023) （2021LZY023）
沈阳市中青年科技创新人才培育专项U35拔尖青年项目(RC230846) （RC230846）
