烟台大学学报（自然科学与工程版）2025，Vol.38Issue(1)：47-54,8.DOI:10.13951/j.cnki.37-1213/n.231103
苹果溶质转运蛋白家族MdSLC25A44-like基因特征和表达分析
Sequence Characteristics and Expression Analysis of MdSLC25A44-like Gene in Malus domestica
摘要
Abstract
In order to study the sequence and expression pattern of the apple solute transport protein family gene MdSLC25A44-like in Malus domestica,the sequence characteristics and tissue expression patterns in the peel and pulp of different cultivars are analyzed using qRT-PCR and bioinformatics analysis.The results show that Md-SLC25A44-like protein has the highest homology with PbSLC25A44-like from white pear.Phylogenetic analysis shows it has the closest affinity to white pear and autumn pear × Western pears.The bioinformatics analysis results indicate that the MdSLC25A44-like protein is an α-Spiral transporter protein,stable protein,and hydrophobic,lo-cated in mitochondria.The results of anthocyanin determination show that the content of anthocyanins in the red skin and red flesh of apples is higher than that in the green skin and white flesh.The real-time fluorescence quanti-tative PCR results show that the expression level of MdSLC25A44-like gene is higher in the red skin and red flesh of apples than in the green skin and white flesh.The above results indicate that the MdSLC25A44-like gene may be in-volved in the process of anthocyanin accumulation,and the expression of the gene is positively correlated with the amount of anthocyanin accumulation.This result provides a scientific basis for further research on the accumulation and transportation of anthocyanins in apple fruits and for improving fruit color quality.关键词
苹果/MdSLC25A44-like基因/序列特征,表达分析,花青苷Key words
apple/MdSLC25A44-like gene/sequence characteristics/expression analysis/anthocyanin分类
园艺学与植物营养学引用本文复制引用
申亚楠,张雪珂,孙晓慧,秦鑫蓉,赵玲玲,宋来庆,刘学卿..苹果溶质转运蛋白家族MdSLC25A44-like基因特征和表达分析[J].烟台大学学报（自然科学与工程版）,2025,38(1):47-54,8.基金项目
烟台市科技发展计划项目(2022XCZX093) （2022XCZX093）
山东省农业良种工程项目(2021LZGC021) （2021LZGC021）
山东省外专双百计划项目(WST2018013). （WST2018013）
