烟台大学学报（自然科学与工程版）2025，Vol.38Issue(1)：64-73,10.DOI:10.13951/j.cnki.37-1213/n.240306
垂穗披碱草中黄酮类化合物的分离及其对高脂血症斑马鱼脂质代谢的影响
Isolation of Flavonoids from Elymus nutans Griseb and Their Effects on Lipid Metabolism in Hyperlipidemic Zebrafish
摘要
Abstract
The aim of this paper is to isolate flavonoids from Elymus nutans Griseb.and to investigate their effects on lipid metabolism in hyperlipidemia zebrafish induced by high-fat diet.Medium-pressure and high-pressure liquid chromatography combined with two-dimensional liquid chromatography were used for the isolation and purification of the compounds.The structures of the compounds were identified using nuclear magnetic resonance spectroscopy(NMR)and mass spectrometry(MS).A hyperlipidemia model of juvenile zebrafish induced by high-fat diet was established.After continuous feeding of each compound for 5 days,changes in the survival rates,body weights,lengths,and BMI of zebrafish larvae in each group were detected.Oil-red O staining and HE staining were respec-tively used to observe changes in lipid accumulations and histopathology.Biochemical kits were used to determine total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-c),and high-density lipopro-tein cholesterol(HDL-c).Real-time fluorescence quantitative PCR(qRT PCR)was used to detect changes in the expression of lipid metabolism-related mRNA.The results show that,two flavonoids isolated from E.nutans Griseb.were identified as schaftoside and isoschaftoside,which were a pair of isomers.Both schaftoside and isos-chaftoside significantly relieved high-fat diet induced liver lipid accumulation and the loosening of liver cell arrange-ment and vacuolization in zebrafish.Compared with the model group,schaftoside and isoschaftoside reduced the content of TG,TC,LDL-c,and increased the content of HDL-c,with the improvement effect of isoschaftoside be-ing more significant.The mechanism of isoschaftoside may be related to down-regulating the expression of fatty acid synthesis genes SREBF1 and FASN,and increasing the expression of cholesterol metabolism-related genes RORα.关键词
中高压液相色谱/夏佛塔苷/异夏佛塔苷/斑马鱼/高脂血症Key words
medium-pressure and high-pressure liquid chromatography/schaftoside/isoschaftoside/zebrafish/hy-perlipidemia分类
中医学引用本文复制引用
童莹莹,初明,党军,黄海辉,马成俊,李刚..垂穗披碱草中黄酮类化合物的分离及其对高脂血症斑马鱼脂质代谢的影响[J].烟台大学学报（自然科学与工程版）,2025,38(1):64-73,10.基金项目
山东省自然科学基金资助项目(ZR2020MH380) （ZR2020MH380）
烟台市科技创新发展计划项目(2021XDHZ078). （2021XDHZ078）
