烟台大学学报（自然科学与工程版）2025，Vol.38Issue(1)：101-109,9.DOI:10.13951/j.cnki.37-1213/n.231112
基于UHPLC-Q-Orbitrap HRMS和生物活性比较分析栀子不同部位的差异
Differences of Different Parts of Gardenia jasminoides Ellis Based on UHPLC-Q-Orbitrap HRMS and Biological Activity Comparison
摘要
Abstract
The UHPLC-Q-Orbitrap HRMS method was used to comprehensively analyze the mass chromatogram of four parts of Gardenia jasminoides Ellis—fruit,flowers,leaves,and roots—to elucidate their chemical composition differences.This analysis was combined with in vitro anti-inflammatory and anti-VSMC proliferation activity experi-ments to explore their medicinal potential.First,a total of 53 compounds were identified from the four parts,inclu-ding iridoid glycosides,terpenoids,polyphenols,flavonoids,organic acid esters,and other compounds.Second,the distribution and content differences of chemical components in the four parts of G.jasminoides Ellis were dis-played through visualization analysis using Venn plot and heatmaps.Finally,the pharmacological activity of extracts from the four parts was evaluated based on their effects on LPS-induced NO production in RAW 264.7 cells and PDGF-BB-induced VSMC proliferation.The results showed significant differences in the chemical composition of the fruits,flowers,leaves,and roots.The diterpenoid compounds,such as crocin glycosides in the fruits,are u-nique.Compared with other parts,the types and contents of components in the fruit and flowers of G.jasminoides Ellis are similar.Through comprehensive activity experiments,the anti-inflammatory and anti-VSMC activities of the G.jasminoides flower were found to be superior to those of the traditional medicinal parts.关键词
栀子/不同部位/UHPLC-Q-Orbitrap HRMS/生物活性Key words
G.jasminoides Ellis/different parts/UHPLC-Q-Orbitrap HRMS/biological activity分类
医药卫生引用本文复制引用
杨晓丽,姜晓雪,徐若瑄,张燕艳,刘荣霞..基于UHPLC-Q-Orbitrap HRMS和生物活性比较分析栀子不同部位的差异[J].烟台大学学报（自然科学与工程版）,2025,38(1):101-109,9.基金项目
国家自然科学基金资助项目(81973513). （81973513）
