医学信息2025，Vol.38Issue(4)：1-9,9.DOI:10.3969/j.issn.1006-1959.2025.04.001
cpsG基因影响神经胶质瘤细胞转录组学分析
Transcriptome Analysis of cpsG Gene Affecting Glioma Cells
摘要
Abstract
Objective To investigate the effect of cpsG gene on the transcriptome of glioma cell line U87MG.Methods Lentivirus rLV-cpsG was infected with human glioma cell U87MG,and the stable cell line was screened.Total RNA was extracted and analyzed by high-throughput RNA sequencing.It included differential gene analysis,GO analysis,KEGG analysis,Reactome analysis,DO analysis,variable shear analysis of differential genes,etc.Results High-throughput RNA sequencing technology showed that cpsG affected gene transcription ability of U87MG cells,and upregulated genes included TMEM132A,CRAMP1,PRSS3,MAP4K3,SEMA3B,etc;down-regulated genes included HOXA11,NFYA,ICA1,SLC7A2,SOX8 and so on.KEGG analysis showed that upregulated KEGG pathways mainly included PI3K-Akt signaling pathway,Toll-like receptor signaling pathway,TNF signaling pathway,NF-kappa B signaling pathway,etc;the down-regulated KEGG pathways mainly included Wnt signaling pathway,MAPK signaling pathway,cAMP signaling pathway,Hippo signaling pathway,etc.Reactome analysis showed that the up-regulated Reactome pathways mainly included extracellular matrix organization,protein metabolism,hemostasis,immune system,etc;the down-regulated Reactome pathways mainly included nervous system,biological development,signal transduction,muscle contraction,etc.DO analysis showed that the up-regulated DO pathway mainly included squamous cell carcinoma,chest cancer,genital organ cancer,adenocarcinoma,gastric cancer,etc;the down-regulated DO pathway mainly includes central nervous system cancer,psychiatric disorders,cognitive disorders,and ovarian clear cell adenocarcinoma.Variable shear analysis showed that SE accounted for 65.97%,MXE accounted for 8.07%,A5SS accounted for 8.68%,RI accounted for 6.82%,and A3SS accounted for 10.46%.Conclusion cpsG gene regulates the transcriptomics of glioma cells,which provides a theoretical basis for the clinical diagnosis and treatment of human glioma.关键词
cpsG/神经胶质瘤细胞/转录组学/RNA测序Key words
cpsG/Glioma cells/Transcriptomics/RNA sequencing分类
医药卫生引用本文复制引用
张陈,詹为易,郭秋麟,喻越,李艳娜..cpsG基因影响神经胶质瘤细胞转录组学分析[J].医学信息,2025,38(4):1-9,9.基金项目
同济大学大学生创新创业训练计划项目(编号:X2022008) （编号:X2022008）
