应用化学2025，Vol.42Issue(2)：182-191,10.DOI:10.19894/j.issn.1000-0518.240391
金纳米棒自组装柔性膜的制备及比率型表面增强拉曼散射细菌传感器
Preparation of Gold Nanorod Self-Assembled Flexible Films and Ratiometric Surface-Enhanced Raman Scattering Detection of Bacteria
摘要
Abstract
Bacteria are key factors in causing infections,and quantitative detection for bacteria is crucial for clinical monitoring and treatment guidance.Gold nanorods(Au NRs)were self-assembled into dense metallic films using a liquid-liquid interface method and successfully transferred onto an ultrathin thermoplastic polyurethane surface,forming a uniform and stable flexible surface-enhanced Raman scattering(SERS)active substrate.This substrate was modified with 4-mercaptophenylboronic acid(MPBA)to provide a reference signal for bacterial fixation.On this substrate,the detection limit for MPBA molecules can reach 1×10-9 mol/L.A gold-silver core-shell structured SERS tag was prepared,using 5,5'-dithiobis(2-nitrobenzoic acid)(DTNB)in the interlayer as the target signal.The signal of SERS tags can be enhanced by about 54 times by the prepared substrate.By combining SERS tags with nucleic acid aptamers,specific recognition of Escherichia coli(E.coli)was achieved.By analyzing the ratio of Raman characteristic peak intensities between the target signal and the reference signal(I1337/I1078),a good linear relationship was observed between bacterial concentration in the range of 1×102 to 1×107 CFU/mL and I1337/I1078 in water and saliva samples,with a coefficient of determination(R2=0.97126).It was superior to analyzing the target signal alone(R2=0.87046),demonstrating that ratiometric analysis provided a better fit.This strategy shows potential application value in bacterial detection.关键词
二液相成膜法/金纳米棒/表面增强拉曼散射/4-巯基苯硼酸/比率型分析Key words
Liquid-liquid interface method/Gold nanorods/Surface-enhanced Raman scattering/4-Mercaptophenylboronic acid/Ratiometric analysis分类
化学引用本文复制引用
田璐璐,胡松涛,屈栩州,王林,董彪..金纳米棒自组装柔性膜的制备及比率型表面增强拉曼散射细菌传感器[J].应用化学,2025,42(2):182-191,10.基金项目
国家自然科学基金面上项目(No.52272080)和吉林省自然科学基金(No.20220402005GH)资助 Supported by the National Natural Science Foundation of China(No.52272080)and the Natural Science Foundation of Jilin Province(No.20220402005GH) （No.52272080）
