应用化学2025，Vol.42Issue(2)：192-200,中插1-中插3,12.DOI:10.19894/j.issn.1000-0518.240220
一种近红外快速响应型硫化氢荧光探针的合成及细胞成像
Synthesis and Cell Imaging of a Near-Infrared Fast Responsive Hydrogen Sulfide Fluorescent Probe
摘要
Abstract
Based on the formation of CuS,a fluorescent probe of H2 S containing BODIPY,Mito-BPN-Cu,was designed and synthesized.The study of photophysical properties show that the maximum absorption peak and the maximum emission wavelength are 694 nm and 805 nm respectively.Mito-BPN-Cu shows high selectivity for hydrogen sulfide and has an extremely fast response speed(within 10 seconds).In both pure acetonitrile and V(acetonitrile)∶V(water)=1∶1 solvent systems,Mito-BPN-Cu has high sensitivity to H2 S,and the detection limit in the latter solvent system reaches 0.56 μmol/L.Mito-BPN-Cu showed low cytotoxicity,and the survival rate of HCT116 cells was still greater than 80%at 48 h and even the concentration of the probe was 150 μmol/L.In addition,the probe can specifically target mitochondria with a Pearson correlation coefficient of 0.92.In view of the above advantages of the probe,Mito-BPN-Cu was successfully used for fluorescence imaging of hydrogen sulfide in HCT116 cells.关键词
硫化氢/荧光探针/荧光成像/线粒体靶向/近红外Key words
Hydrogen sulfide/Fluorescent imaging/Mitochondrial targetable/Near-infrared分类
化学引用本文复制引用
高长江,黄鑫,田亚洋,余海峰,王小波..一种近红外快速响应型硫化氢荧光探针的合成及细胞成像[J].应用化学,2025,42(2):192-200,中插1-中插3,12.基金项目
咸宁市自然科学基金(No.2023ZRKX087)资助 Supported by Xianning Natural Science Foundation(No.2023ZRKX087) （No.2023ZRKX087）
