现代畜牧科技Issue(2)：24-26,3.DOI:10.19369/j.cnki.2095-9737.2025.02.008
骆驼布鲁氏菌病血清学检测方法比较
Comparison of Serological Testing Methods for Camel Brucellosis
摘要
Abstract
In order to enhance the detection of camel brucellosis,five methods,namely Indirect Enzyme-linked Immunosorbent Assay(iELISA),Competitive Enzyme-linked Immunosorbent Assay(cELISA),Rose Bengal Test(RBT),Gold Immunochromatographic Assay(GICA),and Serum Agglutination Test(SAT),were applied to detect camel serum samples,aiming to select appropriate testing methods for practical production.The results indicate that the Kappa values between GICA and cELISA,as well as iELISA and cELISA,are both greater than 0.7,showing good consistency.Compared with SAT,RBT has the highest sensitivity,specificity,and agreement rate,and the lowest false positive and negative rates.Therefore,it is recommended that in large-scale camel brucellosis testing,RBT could be initially used for screening followed by SAT or cELISA for retesting.To reduce the misdiagnosis and missed diagnosis rates of camel brucellosis,it is suggested to combine multiple serological methods for testing to improve diagnostic accuracy.关键词
布鲁氏菌病/血清学/检测Key words
Brucellosis/serology/detection分类
畜牧业引用本文复制引用
段真真,李佳,夏克热木·阿布力孜,迪力夏提·吐尔洪,何宗霖..骆驼布鲁氏菌病血清学检测方法比较[J].现代畜牧科技,2025,(2):24-26,3.基金项目
新疆维吾尔自治区科技特派员科技创业行动计划(2023KY018) （2023KY018）
阿克苏地区科技兴阿项目(2023KJXA011) （2023KJXA011）
