现代畜牧科技Issue(2)：75-77,3.DOI:10.19369/j.cnki.2095-9737.2025.02.022
青海牦牛精子活力与冷冻保存技术的关系研究
Research on the Relationship between Spermatozoa Viability and Cryopreservation Technology in Qinghai Yaks
摘要
Abstract
Qinghai yak(Bos grunniens)is an important economic source for local livestock husbandry,and its reproductive ability has an important impact on local livestock production.However,there are certain difficulties in the technique of yak sperm cryopreservation,especially the significant decrease in sperm viability during the freezing process.In order to investigate the problem of decreased sperm viability during cryopreservation in Qinghai yaks,semen samples from healthy Qinghai yaks were used and treated with various cryoprotectants,such as glycerol,dimethyl sulfoxide(DMSO),and ethylene glycol,respectively,and preserved by fast freezing and slow freezing techniques.Finally,sperm viability was assessed by detecting sperm viability,acrosomal protease activity,and combined fertilization rate.The results showed that glycerol as a cryoprotectant had the best effect on sperm viability,while the slow freezing technique was significantly better than the rapid freezing technique.The optimized cryopreservation technique can effectively improve the sperm viability and fertilization ability of Qinghai yaks,which in turn provides a scientific basis for artificial insemination and large-scale breeding of Qinghai yaks.关键词
青海高原牦牛/精子活力/冷冻保存/养牛业Key words
Qinghai plateau yak/sperm motility/cryopreservation/cattle industry分类
农业科技引用本文复制引用
李景..青海牦牛精子活力与冷冻保存技术的关系研究[J].现代畜牧科技,2025,(2):75-77,3.基金项目
重大科技专项-农村处(青海省科技计划项目):牦牛、藏羊遗传资源挖掘与创新利用(2021-NK-A5) （青海省科技计划项目）
