现代畜牧科技Issue(2)：127-131,5.DOI:10.19369/j.cnki.2095-9737.2025.02.037
miRNA表达水平检测技术研究进展
Advances in the Detection Technique of miRNA Expression Level
摘要
Abstract
miRNAs(microRNAs)are small non-coding RNA molecules approximately 21 to 23 nucleotides in length.They primarily regulate target gene expression by binding to the 3′untranslated region(3′UTR)of target mRNA sequences,often suppressing the translation process.Extensive research has demonstrated that miRNAs play crucial regulatory roles in various biological activities.Consequently,developing miRNA detection technologies with high specificity and sensitivity has become a critical focus.This review provides a comprehensive overview of current miRNA detection methods,including Northern blot,quantitative real-time PCR(qRT-PCR),fluorescence in situ hybridization(FISH),and miRNA sequencing.关键词
miRNA检测/Northern blot/qRT-PCR/FISH/miRNA测序法Key words
miRNA detection/Northern blot/qRT-PCR/FISH/miRNA sequencing分类
生物科学引用本文复制引用
周兆瑞,徐梦思,甘尚权..miRNA表达水平检测技术研究进展[J].现代畜牧科技,2025,(2):127-131,5.基金项目
国家自然科学基金面上项目"miR-SELENBP1介导KRTAP6家族基因表达影响羊毛周期性生长与细度性状的机制研究"(32472889) （32472889）
兵团英才青年项目(2023-2025) （2023-2025）
