中国当代儿科杂志2025，Vol.27Issue(3)：366-372,7.DOI:10.7499/j.issn.1008-8830.2411071
新生大鼠早产儿脑白质损伤的多模态磁共振成像评估及机制探讨
Multi-modal magnetic resonance imaging assessment and mechanism exploration of preterm white matter injury in neonatal rats
摘要
Abstract
Objective To evaluate preterm white matter injury(PWMI)in neonatal rats using multimodal magnetic resonance imaging(MRI)combined with histological assessments and to explore its underlying mechanisms.Methods Healthy 3-day-old Sprague-Dawley neonatal rats were randomly divided into a sham operation group and a PWMI group(n=12 in each group).A PWMI model was established in neonatal rats through hypoxia-ischemia.Laser speckle imaging was used to observe changes in cerebral oxygen saturation and blood flow at different time points post-modeling.Multimodal MRI was employed to assess the condition of white matter injury,while hematoxylin-eosin staining was utilized to observe morphological changes in the striatal area on the injured side.Immunofluorescence staining was performed to detect the proliferation and differentiation of oligodendrocyte precursor cells.Results At 0,6,12,24,and 72 hours post-modeling,the relative blood flow and relative oxygen saturation on the injured side in the PWMI group were significantly lower than those in the sham operation group(P<0.05).At 24 hours post-modeling,T2-weighted imaging showed high signals in the white matter of the injured side in the PWMI group,with relative apparent diffusion coefficient values and Lorenz differential values being lower than those in the sham operation group(P<0.001);additionally,the arrangement of nerve cells in the PWMI group was disordered,and the number of EdU+PDGFR-α+cells was higher than that in the sham operation group(P<0.001).At 28 days post-modeling,the relative fractional anisotropy values,the number of EdU+Olig2+cells,and the fluorescence intensity of myelin basic protein and neurofilament protein 200 in the white matter region of the PWMI group were all lower than those in the sham operation group(P<0.001).Conclusions Multimodal MRI can evaluate early and long-term changes in PWMI in neonatal rat models in vivo,providing both imaging and pathological evidence for the diagnosis and treatment of PWMI in neonates.Hypoxia-ischemia inhibits the proliferation and differentiation of oligodendrocyte precursor cells in neonatal rats,leading to PWMI.关键词
早产儿脑白质损伤/多模态磁共振成像/少突胶质祖细胞/新生大鼠Key words
Preterm white matter injury/Multimodal magnetic resonance imaging/Oligodendrocyte precursor cell/Neonatal rat引用本文复制引用
高啸天,张海默,张孝祖,王怡静,毕惠宁,于淼,李艳,王晓莉..新生大鼠早产儿脑白质损伤的多模态磁共振成像评估及机制探讨[J].中国当代儿科杂志,2025,27(3):366-372,7.基金项目
国家自然科学基金(82071888) （82071888）
山东省自然科学基金(ZR2024MH067) （ZR2024MH067）
2019年高等学校青创人才引育计划 （）
潍坊市科学技术发展计划项目(2021GX058). （2021GX058）
