中国骨伤2025，Vol.38Issue(2)：195-203,9.DOI:10.12200/j.issn.1003-0034.20240924
虎杖苷调节JAK2/STAT3信号通路治疗大鼠激素性股骨头坏死的实验研究
Regulation of JAK2/STAT3 signaling pathway by polydatin in the treatment of hormone-induced femoral head necrosis in rats
摘要
Abstract
Objective To explore the therapeutic effect of polygonum cuspidatum glycoside on steroid-induced osteonecro-sis of the femoral head(SONFH)in rats and its potential mechanism of protecting bone tissue by regulating the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway(JAK2/STAT3).Methods Fifty male SD rats were random-ly divided into control group,model group,low-dose polygonum cuspidatum glycoside group(polygonum cuspidatum glyco-side-L),high-dose polygonum cuspidatum glycoside group(polygonum cuspidatum glycoside-H),and polygonum cuspidatum glycoside-H+Colivelin(JAK2/STAT3 pathway activator)group.SONFH model was induced by lipopolysaccharide and dex-amethasone.The treatment groups were given polygonum cuspidatum glycoside orally(polygonum cuspidatum glycoside-L 10 mg·kg-1,polygonum cuspidatum glycoside-H 20 mg·kg-1,and the polygonum cuspidatum glycoside-H+Colivelin group was injected with Colivelin(1 mg·kg-1)intraperitoneally once a day,while the control and model groups were given an equal vol-ume of saline for 6 weeks.The observed indicators included serum calcium(Ca),serum phosphorus(P),alkaline phosphatase,and transforming growth factor β1(TGF-β1)levels,micro-CT scanning,hematoxylin-eosin staining,and Western blot detec-tion of JAK2/STAT3 signaling pathway and osteogenic differentiation marker genes,including Runt-related transcription factor 2(Runx2),bone morphogenetic protein 2(BMP2),and osteopontin(OPN)protein expression.Results Compared with the model group,the trabecular bone area percentage in the polygonum cuspidatum glycoside-L and polygonum cuspidatum glyco-side-H groups was significantly increased,and the empty lacunar rate was significantly decreased(P＜0.05).Micro-CT analy-sis showed that the bone volume fraction,trabecular number,and thickness increased,and the trabecular separation decreased in the polygonum cuspidatum glycoside-treated groups(P＜0.05).Serum biochemical tests found that the serum Ca and P con-centrations in the polygonum cuspidatum glycoside-L and polygonum cuspidatum glycoside-H groups were restored,the alka-line phosphatase levels decreased,and the transforming growth factor β1 levels increased(P＜0.05).Western blot analysis showed that polygonum cuspidatum glycoside significantly inhibited the activation of the JAK2/STAT3 signaling pathway in the model group and promoted the expression of osteogenic differentiation marker genes such as Runx2,BMP2,and OPN(P＜0.05).Compared with the polygonum cuspidatum glycoside-H group,the improvements in the polygonum cuspidatum glyco-side-H+Colivelin group were somewhat weakened,indicating the importance of the JAK2/STAT3 signaling pathway in the ac-tion of polygonum cuspidatum glycoside.Conclusion polygonum cuspidatum glycoside promotes osteogenic differentiation,im-proves bone microstructure,and has significant therapeutic effects on rat SONFH by regulating the JAK2/STAT3 signaling pathway.关键词
虎杖苷/JAK2/STAT3信号通路/激素性股骨头坏死/成骨分化/骨组织保护Key words
Polygonum cuspidatum glycoside/JAK2/STAT3 signaling pathway/Steroid-induced osteonecrosis of the femoral head/Osteogenic differentiation/Bone tissue protection分类
医药卫生引用本文复制引用
杨祥军,王丛悦,徐西林,胡海,申意伟,张晓峰..虎杖苷调节JAK2/STAT3信号通路治疗大鼠激素性股骨头坏死的实验研究[J].中国骨伤,2025,38(2):195-203,9.基金项目
黑龙江省中医药科研项目(编号:2023327)Heilongjiang Province Traditional Chinese Medicine Scientific Research Project(No.2023327) （编号:2023327）
