中国临床药理学与治疗学2025，Vol.30Issue(2)：216-221,6.DOI:10.12092/j.issn.1009-2501.2025.02.008
杜鹃花总黄酮对脑缺血再灌注大鼠脑氧化应激损伤的改善作用
Ameliorating effect of total flavonoids of rhododendron on brain oxi-dative stress injury in cerebral ischemia-reperfusion rats
摘要
Abstract
AIM:To explore the protective effect of total flavonoids from rhododendron(TFR)on oxi-dative stress injury in the brain of rats subjected to cerebral ischemia/reperfusion(I/R).METHODS:For-ty male SD rats were randomly divided into 5 groups:sham operation group(Sham group),cere-bral ischemia-reperfusion group(MCAO group),and Cerebral Ischemia/Reperfusion with TFR treat-ment groups(TFR 50,100,200 mg/kg groups).The MCAO group and TFR-treated groups underwent ischemia/reperfusion surgery,and the TFR-treated groups received TFR intragastrically for 14 consecu-tive days following the ischemia/reperfusion injury.After 14 days,comparisons were made in terms of neurological function scores,serum inflammatory factors,oxidative stress indicators,and brain injury markers.Additionally,histological examination of brain tissue morphology using Hematoxylin and Eo-sin(HE)staining,observation of cerebral blood flow through cerebral blood flow imaging,and measurement of lactate dehydrogenase(LDH),neu-ron-specific enolase(NSE)activity in serum,inter-leukin-1(IL-1),interleukin-6(IL-6)levels,superox-ide dismutase(SOD)activity,malondialdehyde(MDA)content,nitric oxide(NO)content,and nitric oxide synthase(NOS)activity were performed.RE-SULTS:Compared to the Sham group,MCAO rats exhibited abnormal neurological function scores,severe damage to the microstructure of brain tis-sue,noticeable changes in brain morphology,ele-vated activities of LDH and NSE,increased levels of IL-1 and IL-6,elevated MDA content,and de-creased SOD,NOS activity,and NO content.In com-parison to the MCAO group,rats treated with TFR at doses of 50,100,and 200 mg/kg showed recov-ery of abnormal neurological function scores,re-duced damage to the microstructure of brain tis-sue,decreased activities of LDH and NSE,lowered levels of IL-1 and IL-6,reduced MDA content,and increased SOD,NOS activity,and NO content.CON-CLUSION:Total flavonoids from Rhododendron can protect against cerebral ischemia/reperfusion inju-ry,reducing oxidative stress levels.关键词
杜鹃花总黄酮/脑缺血再灌注/脑缺血缺氧性损伤/氧化应激Key words
total flavonoids of rhododendron/ce-rebral ischemia reperfusion/cerebral ischemic hy-poxic injury/oxidative stress分类
药学引用本文复制引用
余孝海,金宇,孙敏琼,从辉,郭欠影..杜鹃花总黄酮对脑缺血再灌注大鼠脑氧化应激损伤的改善作用[J].中国临床药理学与治疗学,2025,30(2):216-221,6.基金项目
安徽高校科学研究项目(2023AH053310) （2023AH053310）
安徽省研究生教育质量工程项目(2022xscx053) （2022xscx053）
安徽医科大学国家级创新创业项目(202310366037) （202310366037）
