中国临床医学影像杂志2025，Vol.36Issue(3)：153-157,5.DOI:10.12117/jccmi.2025.03.001
缺血缺氧性脑损伤后Glu-CEST成像可视化评估谷氨酸含量及其相关蛋白表达的研究
Glu-CEST imaging for visualized evaluation of glutamate content and related protein expression following hypoxic-ischemic brain injury
摘要
Abstract
Objective:To validate the role and mechanism of glutamate(Glu)metabolism and its transporters/receptors in hypoxic-ischemic brain injury(HIBI)using Glu-CEST imaging.Methods:Twenty-four neonatal pigs(3～5 days old,weighing 1～1.5 kg)were randomly divided into a control group(n=4)and a HIBI group(n=20).The HIBI group was further divided into subgroups based on the time after HIBI(0～6 h,6～12 h,12～24 h,24～48 h,48～72 h;n=4 per subgroup).Changes in Glu levels in the basal ganglia at different time points after HIBI were detected using 7.0T MR Glu-CEST imaging.The expression of Glu transporters/receptors(vGLUT1,NMDAR2A,mGluR2+3)was observed using Western blot,and relevant correlations were analyzed.Statistical significance was set at P<0.05.Results:Compared with the control group,the Glu-CEST effect in the HIBI group exhibited a"double-peak"pattern,with peaks at 6～12 h and 24～48 h(P<0.001).Compared with the control group,vGLUT1 expression increased at 0～6 h after HIBI,slightly decreased at 6～12 h,increased again and reached its peak at 12～24 h(P<0.001),and then decreased.NMDAR2A expression significantly decreased at 0～6 h,increased thereafter,and decreased again at 24～48 h(P<0.001).The expression of mGluR2+3 gradually increased after HIBI,peaked at 12～24 h(P<0.001),and then decreased subsequently.Correlation analysis showed that the Glu-CEST effect was negatively correlated with NMDAR2A expression(r=-0.514,P=0.001).Additionally,vGLUT1 expression was positively correlated with mGluR2+3 expression(r=0.556,P=0.005),and NMDAR2A was positively correlated with mGluR2+3 expression during the 0～48 h period after HIBI(r=0.6442,P=0.007).Conclusion:Glu-CEST imaging can visually assess changes and regional differences in Glu levels in the brain following HIBI in neonatal pigs.The dynamic changes in Glu-related protein expression after HIBI may reveal the regulatory mechanism of excitotoxic brain injury.关键词
缺氧缺血,脑/脑损伤/磁共振成像Key words
Hypoxia-Ischemia,Brain/Brain Injuries/Magnetic Resonance Imaging分类
临床医学引用本文复制引用
赵思嘉,张琴,郑阳..缺血缺氧性脑损伤后Glu-CEST成像可视化评估谷氨酸含量及其相关蛋白表达的研究[J].中国临床医学影像杂志,2025,36(3):153-157,5.基金项目
国家自然科学基金青年基金项目(81801658) （81801658）
辽宁省科技计划联合计划项目(No.2024-MSLH-565). （No.2024-MSLH-565）
