中国临床医学影像杂志2025，Vol.36Issue(3)：158-161,167,5.DOI:10.12117/jccmi.2025.03.002
基于GABA-CEST成像新生猪缺血缺氧脑损伤后GABA代谢的相关研究
Expression changes in GABA metabolism after hypoxic-ischemic brain injury:evidence of 7.0T GABA-CEST imaging
摘要
Abstract
Objective:To investigate the dynamic changes and metabolism of GABA in the basal ganglia of a neonatal pig model of hypoxic-ischemic brain injury(HIBI)using 7.0T GABA-CEST imaging,aiming to clarify the self-regulation of GABA metabolism and its mechanisms in HIBI.Methods:Healthy neonatal pigs aged 3 to 5 days were selected,with 5 pigs in the control group and 24 pigs in the HIBI model group.The model group was further divided into 6 subgroups(n=4):0～2 h,2～6 h,6～12 h,12～24 h,and 24～48 h.GABA-CEST imaging was performed to detect changes in GABA levels in the basal ganglia at different time points following HIBI.Western blot analysis was conducted to assess the expression of receptors(GABRG2),synthesizing enzymes(GAD65,GAD67),and transporters(GAT1,GAT3).Results:After HIBI,the expressions of GABA and GABRG2 showed an initial increase followed by a decrease,with GABA peaking at 24～48 h and GABRG2 reaching its highest level at 0～2 h.In the early phase(0～2 h)following HIBI,the patterns of GAD expression were opposite;GAD65 increased while GAD67 decreased.Both GAD65 and GAD67 subsequently increased before declining between 12～24 h and 24～48 h,aligning with the changes observed in GABA.The expression of GAT1 differed from the control group only at 2～6 h,6～12 h,and 24～48 h,showing a decrease compared to the control group.In contrast,GAT3 demonstrated an overall trend of first rising and then falling,peaking at 6～12 h,earlier than GABA.Conclusion:The results indicate that the 7.0T GABA-CEST imaging technique can non-invasively and quantitatively reflect changes in GABA concentration in the basal ganglia of newborn pigs following HIBI.The observed changes in the expression of GABRG2,GAT,and GAD suggest that GABA may initially act as a neurotransmitter to counteract excitotoxicity and may later serve as an energy substrate to support neuronal activity.关键词
缺氧缺血,脑/脑损伤/磁共振成像Key words
Hypoxia-Ischemia,Brain/Brain Injuries/Magnetic Resonance Imaging分类
临床医学引用本文复制引用
张琴,赵思嘉,郑阳..基于GABA-CEST成像新生猪缺血缺氧脑损伤后GABA代谢的相关研究[J].中国临床医学影像杂志,2025,36(3):158-161,167,5.基金项目
国家自然科学基金青年基金项目(81801658) （81801658）
辽宁省科技计划联合计划项目(No.2024-MSLH-565). （No.2024-MSLH-565）
