中国美容医学2025，Vol.34Issue(3)：7-13,7.
心理应激对SD大鼠颞下颌关节髁突软骨细胞凋亡及Caspase-3表达的影响
The Effects of Psychological Stress on Apoptosis of Condylar Chondrocytes in Temporomandibular Joint and Expression of Caspase-3 in Rats
郑程东 1张媛媛 2张海娟 1韩妍冬 3于传垒 3陈鹤玲 1王爽1
作者信息
- 1. 西安交通大学口腔医院陕西省颅颌面精准医学研究重点实验室 陕西 西安 710004||西安交通大学口腔医院陕西省牙颌面疾病临床研究中心 陕西 西安 710004||西安交通大学口腔医院口腔正畸科 陕西 西安 710004
- 2. 可丽尔医疗科技常州有限公司 江苏 常州 213145
- 3. 西安交通大学口腔医院陕西省颅颌面精准医学研究重点实验室 陕西 西安 710004||西安交通大学口腔医院陕西省牙颌面疾病临床研究中心 陕西 西安 710004
- 折叠
摘要
Abstract
Objective This study aimed to explore the effects of simple psychological stress on the apoptosis of condylar chondrocytes and the expression of Caspase-3 in rats.Methods The histological changes in rat TMJ cartilage,chondrocyte apoptosis,and Caspase-3 expression post-stress will be examined using a psychological stress model in rats with HE staining,TUNEL assay,and immunohistochemistry.Results The results showed that compared with the control group,significant behavioral changes were observed in the experimental group rats after 1 week,3 weeks,and 5 weeks of psychological stress(P＞0.05).There was a slight reduction in body weight gain but no statistical difference,open field and elevated tests indicated that the anxiety level of rats in the psychological stress group increased(P＜0.05).Histological analysis found that psychological stress led to damage of cartilage structure and increase in chondrocyte apoptosis rate,which worsened with the extension of stress time(P＜0.05).The expression of Caspase-3 also increased with the extension of stress time,but decreased in the recovery period(P＜0.05).Conclusion This indicates that simple psychological stress can cause pathological changes in the condylar cartilage of the temporomandibular joint,which may be related to increased expression of Caspase-3 in the articular cartilage and excessive apoptosis of chondrocytes.关键词
心理应激/颞下颌关节/颞下颌关节紊乱病/髁突软骨/细胞凋亡/Caspase-3Key words
psychological stress/temporomandibular joint/temporomandibular joint disorder/condylar cartilage/apoptosis/Caspase-3分类
医药卫生引用本文复制引用
郑程东,张媛媛,张海娟,韩妍冬,于传垒,陈鹤玲,王爽..心理应激对SD大鼠颞下颌关节髁突软骨细胞凋亡及Caspase-3表达的影响[J].中国美容医学,2025,34(3):7-13,7.
