中国美容医学2025，Vol.34Issue(3)：13-18,6.
芦荟大黄素抑制人黑素瘤细胞A375增殖和侵袭的作用及机制初探
Inhibitory Effect of Aloe-emodin on Proliferation and Invasion of Human Melanoma Cells A375 Via AKT/mTOR Pathway
摘要
Abstract
Objective To explore the mechanism of aloe emodin inhibiting proliferation and invasion of human melanoma cells(A375)via AKT/mTOR pathway.Methods A375 cell group,paclitaxel group(500 μmol·ml-1),aloe emodin low-dose group(500 μmol·ml-1)and high-dose group(1 000 μmol·ml-1)were set up,and 6 parallel samples were cultured for 72h in each group.After the experiment,the cell proliferation level of each group was measured by tetramethylazolium blue(MTT)method,cell apoptosis rate and cell cycle detected by flow cytometry,cell invasion level determined by Transwell method,and cell migration level was detected by scar assay.The mRNA and protein expression levels of AKT and mTOR were detected by RT-qPCR and Western blot.Results Compared with A375 cell group,the OD value,survival rate,membrane penetration number,migration distance,mRNA and protein expression levels of AKT and mTOR in paclitaxel group,aloe emodin low-dose and high-dose groups were decreased(P＜0.05),while apoptosis rate and G2/M were increased(P＜0.05).Compared with paclitaxel group,the OD value,survival rate,membrane penetration number,migration distance,mRNA and protein expression levels of AKT and mTOR were increased in aloe emodin low-dose group(P＜0.05),while apoptosis rate and G2/M were decreased(P＜0.05).There were no significant changes in OD value,survival rate,membrane penetration number,migration distance,apoptosis rate,G2/M,AKT and mTOR mRNA and protein expression levels in aloe emodin high-dose group(P＞0.05).Compared with aloe emodin low-dose group,the OD value,survival rate,membrane penetration number,migration distance,mRNA and protein expression levels of AKT and mTOR were decreased in aloe emodin high-dose group(P＜0.05),and apoptosis rate and G2/M were decreased(P＜0.05).Conclusion Aloe emodin can significantly inhibit the proliferation and invasion of melanoma cells,and the mechanism may be related to the inhibitory effect of aloe emodin on the activation of AKT/mTOR pathway.关键词
芦荟大黄素/AKT/mTOR途径/人黑素瘤/细胞增殖/细胞侵袭Key words
aloe emodin/AKT/mTOR pathway/human melanoma/cell proliferation/cell invasion分类
医药卫生引用本文复制引用
李妍芯,赵奥林,阮英..芦荟大黄素抑制人黑素瘤细胞A375增殖和侵袭的作用及机制初探[J].中国美容医学,2025,34(3):13-18,6.基金项目
湖北高校省级大学生创新创业训练项目(编号:S202110927030) （编号:S202110927030）
