中国农业科技导报2025，Vol.27Issue(3)：49-59,11.DOI:10.13304/j.nykjdb.2024.0111
大豆扩展蛋白基因GmEXLA1在荚果发育中的功能鉴定
Function Analysis of Soybean Expansin Gene GmEXLA1 in Plant Pod and Seed Development
摘要
Abstract
Expansin is a kind of protein that can expand the plant cells by loosening the cell wall and releasing the intracellular expansion pressure.Expansin plays an important role in plant growth,development and resistances to diverse stresses.However,the role of expansin in pod and seed development remains unclear.In view of this,an expansin gene GmEXLA1 was cloned and analyzed in this study,and the gene function in pod and seed development was studied.The results showed that GmEXLA1 was predominantly expressed in soybean pod and seed,with an open reading frame length of 780 bp which encoding 259 amino acids.The amino acids from 1 to 19 were the signal peptide of the expansin GmEXLA1,and GmEXLA1 possessed 2 conserved domains of expansins which were necessary for their expanding functions.The subcellular localization analysis showed that the expansin GmEXLA1 was localized in plant cell wall.Further analysis showed that the overexpression of GmEXLA1 significantly increased the pod length,width and seed weight of transgenic Arabidopsis,with the seed weight increased by 61.2%～80.6%.On the contrary,the nonsense mutation of GmEXLA1 significantly reduced soybean pod length,seed length,width and weight,with the decreases of seed-length,-width and-weight 13.5%,7.0%and 25.6%,respectively.These results indicated that the expansin gene GmEXLA1 played an important role in plant pod and seed developmental processes.In addition,the allelic variation analysis of GmEXLA1 showed 15 SNPs in different soybean germplasms,with an upstream SNP(A/G)presenting significantly different distribution frequencies between the wild and cultivated soybeans(A85%vs A38%;G15%vs G62%).Above results provided important functional expansin gene for the improvement of soybean yield related traits via the biological breeding technology.关键词
大豆/扩展蛋白/细胞壁/荚果发育/等位变异Key words
soybean/expansin/cell wall/pod and seed development/allelic variation分类
农业科学引用本文复制引用
李娜,张华,邢馨竹,邵振启,杨占武,李喜焕,张彩英..大豆扩展蛋白基因GmEXLA1在荚果发育中的功能鉴定[J].中国农业科技导报,2025,27(3):49-59,11.基金项目
河北省现代农业产业技术体系建设专项(HBCT2024060204). （HBCT2024060204）
