中国农业科技导报2025，Vol.27Issue(3)：71-82,12.DOI:10.13304/j.nykjdb.2023.0591
茶树钙调蛋白结合转录激活因子(CAMTA)家族基因鉴定与表达分析
Identification and Expression Analysis of Calmodulin-binding Transcription Activator(CAMTA)Family Genes in Tea Plants
摘要
Abstract
In order to understand calmodulin-binding transcription activator(CAMTA)family members in tea plants and explore their expression characteristics,the CsCAMTAs were identified in the whole genome of tea plants,and their physicochemical properties,gene structure,domain,conserved motif order,phylogenetic relationship and cis-acting elements were analyzed by bioinformatics method,and the expression of CsCAMTAs in different tissues,under different hormone treatments and bacterial stress were analyzed by qRT-PCR.The results showed that a total of 10 CsCAMTAs members were identified with 10 motifs,which distributed in 6 chromosomes and all localized in the nucleus.The sizes of different CsCAMTAs gene were different with the number of amino acids from 124 to 1 419,the isoelectric points of the coding protein were from 4.976 to 9.820,and only 1 was weak hydrophobic protein,the others were hydrophilic proteins.Phylogenetic analysis showed that the CsCAMTAs were clustered into 4 groups,with 4 homologous gene pairs,and the homologous gene pairs had high similarity in gene structure,conserved domain,number of conserved motifs and relative position.The promoter region of CsCAMTAs contained 34 cis-acting elements associated with light response,hormone induction,and biotic stress.The expression analysis results showed that the expressions of CsCAMTAs in different tissues were significantly different.CsCAMTA1,3,4,7,8,10 had higher expression level in mature leaves.Except for CsCAMTA5,which was not induced by methyl jasmonate,the others were induced by methyl jasmonate,abscisic acid,salicylic acid and tea wheel spot pathogen,which indicated that CsCAMTAs not only participated in the regulation of tea plants growth and development,but also participated in the response of tea plants to bacteria and hormones.关键词
茶树/钙调蛋白结合转录激活因子(CAMTA)/生物信息学分析/表达分析Key words
tea plants/calmodulin-binding transcriptional activator(CAMTA)/bioinformatics analysis/expression analysis分类
农业科学引用本文复制引用
刘金仙,王丽娟,刘杰,傅仙玉,武广珩..茶树钙调蛋白结合转录激活因子(CAMTA)家族基因鉴定与表达分析[J].中国农业科技导报,2025,27(3):71-82,12.基金项目
福建省自然科学基金项目(2022J011200) （2022J011200）
福建省科技计划引导性项目(2023N0017) （2023N0017）
资源化学产业科技创新联合项目(N2021Z006). （N2021Z006）
