中国实验动物学报2025，Vol.33Issue(1)：1-13,13.DOI:10.3969/j.issn.1005-4847.2025.01.001
基于网络药理和动物实验探讨活血通络方治疗脊髓损伤的作用机制
Exploring the mechanism of action of Huoxue Tongluo prescription for the treatment of spinal cord injury based on network pharmacology and molecular docking technology
摘要
Abstract
Objective This study preliminarily investigated the potential mechanisms of the Huoxue Tongluo prescription(HXTLP)in treating spinal cord injury(SCI)through a combination of network pharmacology,molecular docking technology,and in vivo experimental verification.Methods The traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)were utilized to select the active ingredients,targets of action were obtained from Swiss target prediction database,and an"active ingredients-targets"network was constructed.SCI-related targets were obtained by accessing online mendelian inheritance in man(OMIM)and human gene database(GeneCards),and a protein interaction network of the common targets of HXTLP and SCI was established based on the search tool for the retrieval of interacting genes/protein(STRING)database.The Metascape database was used in KEGG pathway enrichment and GO analyses of the common targets.Molecular docking of active ingredients and key targets was performed through Autodock 1.5.7 software,and the results were visualized with Pymol 2.4.0 software.Finally,the effect of HXTLP on SCI was verified by animal experiments.Results A total of 184 intersection targets were obtained,and the key targets were serine/threonine kinase(AKT1),signal transducer and activator of transcription 3(STAT3),heat shock protein 90 kDa alpha,class A member 1(HSP90AA1),phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha(PIK3CA),phosphoinositide-3-kinase regulatory subunit 1(PIK3R1),harvey ras(HRAS),estrogen receptor 1(ESR1),mitogen-activated protein kinase 1(MAPK1),and epidermal growth factor receptor(EGFR).Molecular docking result showed strong binding abilities between the core active components and key targets.In the animal experiments,the behavioral scores of mice in the HXTLP group increased(P＜0.05),the motor function of hind limbs was improved,and the histological morphology of the injured area was more complete compared with those of the model group.Western Blot result revealed that HXTLP effectively inhibited the key target protein(HSP90AA1)and the expression of phospho-STAT3(P-STAT3)and promoted the expression of phospho-phosphatidylinositol-3-kinase(P-PI3K)and phospho-AKT1(P-AKT1).Conclusions This study verified that HXTLP has multi-component,multi-target,and multi-pathway synergistic effects in the treatment of SCI and has provided experimental and theoretical bases for further clinical medication research for SCI.关键词
活血通络方/脊髓损伤/网络药理/分子对接/动物实验Key words
Huoxue Tongluo prescription/spinal cord injury/network pharmacology/molecular docking/animal experiments分类
生物科学引用本文复制引用
王佳星,陈家扬,申婷婷,王智慧,任晓平..基于网络药理和动物实验探讨活血通络方治疗脊髓损伤的作用机制[J].中国实验动物学报,2025,33(1):1-13,13.基金项目
国家自然科学基金(82060874),广西重点研发计划项目(桂科AB21196062),广西中医药大学2023年研究生教育创新计划项目(YCSY2023042).Funded by National Natural Science Foundation of China(82060874),Focus on Research and Development Projects in Guangxi(Guike AB21196062),2023 Graduate Education Innovation Plan of Guangxi University of Chinese Medicine(YCSY2023042). （82060874）
