中国实验动物学报2025，Vol.33Issue(1)：89-98,10.DOI:10.3969/j.issn.1005-4847.2025.01.009
雾化吸入脂多糖导致的大鼠急性肺炎模型不同标本中炎性因子比较研究
Comparative research of inflammatory factors in different specimens from a rat model of aspiration pneumonia induced by lipopolysaccharide
摘要
Abstract
Objective To investigate the changes of inflammatory factors in bronchoalveolar lavage fluid(BALF),lung tissue,and serum of a rat pneumonia model induced by inhalation of lipopolysaccharides(LPS).Methods Three days after modeling by LPS 4 mg/mL inhalation,15 min/d,was conducted while monitoring the particle size distribution and aerosol concentration of LPS,the degree of inflammation in lung tissues of rats in each group was observed via HE staining,and neutrophils in BALF were counted by microscope.The contents of interferon gamma(IFN-γ),interleukin-1 beta(IL-1 β),IL-4,IL-5,IL-6,IL-10,IL-13,tumor necrosis factor alpha(TNF-α),and KC/GRO in lung tissue,serum,and BALF were detected by Meso Scale Discovery.Results The lung histopathology of model rats displayed focal and diffuse alveolar epithelial necrosis with shedding and the aggregation and infiltration of inflammatory cells.The particle size distribution of atomized LPS was as follows,Dv(10)=0.6974μm,Dv(50)=3.387 µm,Dv(90)=8.836 μm.The aerosol concentration of LPS was 4.08 g/m3,and the calculated inhalation dose for rats was 47.10 mg/kg.The neutrophil count(P＜0.01)and contents of IL-1β,IL-6,and TNF-α(P＜0.05,P＜0.001,P＜0.001)in the BALF,and the contents of IL-1β,IL-6,and KC/GRO in lung tissue(P＜0.01,P＜0.05,P＜0.01),of model rats were significantly increased.No biologically significant changes were observed in inflammatory factor levels in the serum.Conclusions In the acute pneumonia model induced by inhalation of LPS,significant changes in inflammatory factors such as IL-1β,IL-6,KC/GRO,and TNF-α were observed in both lung tissue and bronchoalveolar lavage fluid(BALF),while no notable changes in these inflammatory factors were detected in serum.This indicates that the inflammation responses are primarily localized in the lungs.关键词
急性肺炎模型/脂多糖/雾化吸入/炎性因子/白细胞分类计数Key words
acute pneumonia model/lipopolysaccharide/aspiration/inflammatory factors/neutrophils count Conflicts of Interest:The authors declare no conflict of interest分类
生物学引用本文复制引用
宋玲,高云航,李晗,陈腾飞,侯红平,叶祖光,张广平..雾化吸入脂多糖导致的大鼠急性肺炎模型不同标本中炎性因子比较研究[J].中国实验动物学报,2025,33(1):89-98,10.基金项目
中药研究所中药药理创新团队项目(CI2021B015),中国中医科学院创新工程项目(C12021A04615),国家自然科学基金青年项目(82204740).Funded by Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2021B015),Innovation Project of China Academy of Chinese Medical Sciences(C12021A04615),Youth Project of the National Natural Science Foundation of China(82204740). （CI2021B015）
