中国兽医杂志2025，Vol.61Issue(3)：39-43,5.DOI:10.20157/j.cnki.zgsyzz.2025.03.005
西藏日喀则地区羊梨形虫病原调查和分析
Pathogen Investigation and Analysis of Piroplasma Infection in Tibetan Sheep in Shigatse,Tibet,China
摘要
Abstract
To understand the prevalence of ovine piroplasmosis in Tibetan sheep in Shigatse City,Tibet Autonomous Region,366 blood samples from Tibetan sheep were collected,and genomic DNA was extracted.Nested PCR was used to detect and identify the pathogens of ovine piroplasmosis.Additionally,a phylogenetic tree was constructed based on the 18S rRNA gene sequence of piroplasma.The results showed that the piroplasma infection rate was 35.79%(131/366),with the prevalence of Theileria ovis in sheep being 35.25%(129/366)and the prevalence of Theileria annulata being 0.55%(2/366).The phylogenetic tree revealed that Theileria ovis from this region clustered with strains from Xinjiang,China(GenBank:FJ603460),Turkey(GenBank:AY260172),and Sudan(GenBank:AY260171)in the same branch.Theileria annulata clustered with strains from Italy(GenBank:MT341858),Turkey(GenBank:524666),and Inner Mongolia,China(GenBank:EU083801)in the same branch.This study is the first to report the presence of Theileria ovis in Tibet,providing valuable data for the epidemiological survey and integrated prevention and control of ovine piroplasmosis in the region.关键词
藏羊/梨形虫/巢式PCR/系统进化树/流行病学调查Key words
Tibetan sheep/piroplasma/nested PCR/phylogenetic tree/epidemiological investigation分类
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朱浩瀚,赵帅阳,张少华,张楚晗,苏书晓,关贵全,殷宏,罗建勋,刘军龙..西藏日喀则地区羊梨形虫病原调查和分析[J].中国兽医杂志,2025,61(3):39-43,5.基金项目
国家重点研发计划项目(2022YFD1302102-06) （2022YFD1302102-06）
国家自然科学基金项目(32202836,31972706) （32202836,31972706）
国家寄生虫资源库项目(NPRC-2019-194-30) （NPRC-2019-194-30）
农业科技创新工程(ASTIP)(CAAS-ASTIP-2016-LVRI) （ASTIP）
甘肃省基础研究创新群体项目(22JR5RA024) （22JR5RA024）
甘肃省科技厅特派团项目(22CX8NA011) （22CX8NA011）
