中国兽医杂志2025，Vol.61Issue(3)：50-57,8.DOI:10.20157/j.cnki.zgsyzz.2025.03.007
血根碱联合β-内酰胺类抗生素对金黄色葡萄球菌的抗菌作用
Antibacterial Effect of Sanguinarine Combined with β-lactam Antibiotics on Staphylococcus aureus
康艳华 1钟翌芃 2马强 1梁书坤 1钟友刚1
作者信息
- 1. 中国农业大学动物医学院,北京 海淀 100193
- 2. 南京农业大学动物医学院,江苏 南京 210095
- 折叠
摘要
Abstract
To investigate the antibiotic resistance of Staphylococcus aureus(S.aureus)isolated from bovine mastitis against commonly used β-lactam antibiotics,and to explore the synergistic antibacterial effects of sanguinarine(SAN)combined with β-lactam antibiotics on drug-resistant S.aureus,57 strains of S.aureus were isolated from clinical bovine mastitis samples.The minimal inhibitory concentration(MIC)of eight commonly used β-lactam antibiotics was determined by the microdilution broth method.The fractional inhibitory concentration indices(FICI)of SAN and β-lactam antibiotics was determined using the microdilution checkerboard dilution method,and the bactericidal effect of SAN combined with β-lactam antibiotics was tested using the in vitro time-kill curve.The results showed that the resistance rate of S.aureus to β-lactam antibiotics was 59.65%,with severe resistance to ampicillin(40.35%),penicillin G(35.09%),and amoxicillin(35.09%).SAN exhibited synergistic effects with penicillin G,ampicillin,amoxicillin,and cefalexin,reducing the MIC of β-lactam antibiotics by 2-64 times.The time-kill curves indicated that SAN could enhance the bactericidal effect of penicillin G,ampicillin,amoxicillin,and cefalexin on drug-resistant S.aureus.The results suggest that SAN can inhibit the growth of resistant bacteria and restore the antibacterial activity of β-lactam antibiotics against S.aureus,providing scientific reference for the treatment of bovine mastitis caused by S.aureus.关键词
金黄色葡萄球菌/血根碱/β-内酰胺类抗生素/奶牛乳房炎Key words
Staphylococcus aureus/sanguinarine/β-lactam antibiotics/bovine mastitis分类
农业科技引用本文复制引用
康艳华,钟翌芃,马强,梁书坤,钟友刚..血根碱联合β-内酰胺类抗生素对金黄色葡萄球菌的抗菌作用[J].中国兽医杂志,2025,61(3):50-57,8.
