中国兽医杂志2025，Vol.61Issue(3)：110-117,8.DOI:10.20157/j.cnki.zgsyzz.2025.03.015
包载PLGA的蒲公英多糖纳米粒对Raw264.7细胞免疫效果的评价
Evaluation of the Immune Effects of PLGA-Encapsulated Dandelion Polysaccharide Nanoparticles on Raw264.7 Cells
摘要
Abstract
To investigate the immune effects of poly(lactic-co-glycolic acid)(PLGA)-encapsulated Dandelion polysaccharide nanoparticles(PLGA-DP-NP)on RAW264.7 murine macrophage cells,this study used RAW264.7 cells as the research model.The cytotoxicity and proliferation effects of PLGA-DP-NP on RAW264.7 cells were assessed using the CCK-8 assay.The secretion levels of interleukin-2(IL-2)and interleukin-6(IL-6)were measured using an enzyme-linked immunosorbent assay(ELISA).Flow cytometry was employed to examine the expression of the cell maturation markers CD80 and CD86 and the phagocytosis of ovalbumin(OVA)by RAW264.7 cells under low,medium,and high concentrations(18.8,75,and 150 μg/mL)of PLGA-DP-NP.The results showed that RAW264.7 cell viability remained high when the PLGA-DP-NP concentration was below 500 μg/mL.Compared with the Negative control group,PLGA-DP-NP at concentrations of 18.8,37.5,75,150,and 300 μg/mL significantly promoted cell proliferation(P<0.05)and increased the secretion of IL-2 and IL-6(P<0.05).Low,medium,and high concentrations of PLGA-DP-NP enhanced the expression of CD80(P>0.05,P<0.01,or P<0.05)and CD86(P<0.01)in RAW264.7 cells,and increased the percentage of Raw264.7 cells that phagocytose OVA(P<0.01),with the most pronounced effect observed at 75 μg/mL.These findings indicate that PLGA-DP-NP,within a safe concentration range,can significantly promote RAW264.7 cell proliferation,enhance IL-2 and IL-6 secretion,facilitate cell maturation,and improve phagocytic activity,with the optimal effect at 75 μg/mL.关键词
蒲公英多糖/聚乳酸-羟基乙酸共聚物(PLGA)/Raw264.7细胞/免疫效果Key words
Dandelion polysaccharide/poly(lactic-co-glycolic acid)(PLGA)/Raw264.7 cell/immune effect分类
农业科技引用本文复制引用
李向辉,宋幸辉,杨星宇,马霞..包载PLGA的蒲公英多糖纳米粒对Raw264.7细胞免疫效果的评价[J].中国兽医杂志,2025,61(3):110-117,8.基金项目
河南省科技攻关项目(232102110125,242102111034) （232102110125,242102111034）
河南省"兽医"重点学科(312) （312）
河南牧业经济学院博士科研启动资金项目(2022HNUAHEDF026) （2022HNUAHEDF026）
